Difference between revisions of "Part:BBa K590006"

Latest revision as of 06:04, 22 September 2011

mamPA, from Magnetospirillum magneticum

This part includes gene mamP and mamA, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

The genes are AMB0970 and AMB0971 respectively. It was reported that loss of mamP causes weak magnetic response, with large but fewer crystals; mamA is required for magnetosome activation; activation of vesicles

Below is the gel image of mamPA(1493bp):

1kb Ladder (left), mamPA (right)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 399
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 399
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 399
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 399
Illegal NgoMIV site found at 269
Illegal NgoMIV site found at 456
Illegal NgoMIV site found at 1493
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K590006 short</partinfo>
-AMB#0970,0971; Gene function: loss of mamP causes weak magnetic response, with large but fewer crystals; mamA is required for magnetosome activation; activation of vessicles
+This part includes gene mamP and mamA, which were extracted from the genome of ''Magnetospirillum magneticum'' strain AMB-1  by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The genes are AMB0970 and AMB0971 respectively. It was reported that loss of mamP causes weak magnetic response, with large but fewer crystals; mamA is required for magnetosome activation; activation of vesicles
+Below is the gel image of mamPA(1493bp):
+[[Image:Igem2011 PA gel.png|center|thumb|1kb Ladder (left), mamPA (right)]]
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>