Difference between revisions of "Part:BBa K546003"
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<partinfo>BBa_K546003 short</partinfo> | <partinfo>BBa_K546003 short</partinfo> | ||
− | This part was made as a replacement for a defective version of BBa_F2621 | + | This part was made as a replacement for a defective version of BBa_F2621. After sequencing we noticed that BBa_F2621 was lacking the BBa_R0063 promoter. We tested the expression levels of BBa_K546003 and BBa_F2621 using RBS-J04031-B0015 as a reporter (See BBA_K546002). BBa_K546003 had a significantly higher GFP signal than the F2621 BioBrick part we received, this indicated that we fixed it. |
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− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>'''Sequence and Features'''</span> |
<partinfo>BBa_K546003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K546003 SequenceAndFeatures</partinfo> | ||
+ | ==Experimental data== | ||
+ | |||
+ | See [https://parts.igem.org/Part:BBa_K546002 K546002] | ||
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Latest revision as of 21:24, 21 September 2011
Lux pL controlled LuxR + lux pR promoter.
This part was made as a replacement for a defective version of BBa_F2621. After sequencing we noticed that BBa_F2621 was lacking the BBa_R0063 promoter. We tested the expression levels of BBa_K546003 and BBa_F2621 using RBS-J04031-B0015 as a reporter (See BBA_K546002). BBa_K546003 had a significantly higher GFP signal than the F2621 BioBrick part we received, this indicated that we fixed it.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Experimental data
See K546002