Difference between revisions of "Part:BBa K546003"

Latest revision as of 21:24, 21 September 2011

Lux pL controlled LuxR + lux pR promoter.

This part was made as a replacement for a defective version of BBa_F2621. After sequencing we noticed that BBa_F2621 was lacking the BBa_R0063 promoter. We tested the expression levels of BBa_K546003 and BBa_F2621 using RBS-J04031-B0015 as a reporter (See BBA_K546002). BBa_K546003 had a significantly higher GFP signal than the F2621 BioBrick part we received, this indicated that we fixed it.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1101

Experimental data

See K546002

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K546003 short</partinfo>
-This part was made as a replacement for a defective version of BBa_F2621 which lacked the R0063 promoter, but was otherwise intact and functional. It shows a significantly higher response to 3OC6HSL than the incomplete BBa_F2621 (which is present in the Registry as BBa_K317037)
+This part was made as a replacement for a defective version of BBa_F2621. After sequencing we noticed that BBa_F2621 was lacking the BBa_R0063 promoter. We tested the expression levels of BBa_K546003 and BBa_F2621 using RBS-J04031-B0015 as a reporter (See BBA_K546002). BBa_K546003 had a significantly higher GFP signal than the F2621 BioBrick part we received, this indicated that we fixed it.
 <!-- Add more about the biology of this part here
@@ Line 9: / Line 8: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'>'''Sequence and Features'''</span>
 <partinfo>BBa_K546003 SequenceAndFeatures</partinfo>
+==Experimental data==
+See [https://parts.igem.org/Part:BBa_K546002 K546002]
 <!-- Uncomment this to enable Functional Parameter display