Difference between revisions of "Part:BBa K515107:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
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===Applications of BBa_K515107===
 
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<html>
 
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<p><b>Characterisation</b></p>
 
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<p>This part (BBa_K515107) has been characterised in a number of aspects to test its properties as a reporter. The tests describe this part in terms of thermostability, photostability and photoconversion.</p>
 
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<p><b>Thermostability</b></p>
 
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<p></p>
 
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<p><b>Photostability</b></p>
 
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<p></p>
 
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<p><b>Photoconversion</b></p>
 
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<p></p>
 
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<p><b>Photoconversion using confocal microscope</b></p>
 
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<p>This part has been used as a reporter for observation of bacterial uptake into the roots of the plants. Due to its photoconvertible properties, it allows to monitor the metabolic activity of the bacterial cell once uptaken into the root. Dendra was converted from </p>
 
 
===User Reviews===
 
===User Reviews===
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<partinfo>BBa_K515107 AddReview number</partinfo>
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<partinfo>BBa_K515107 AddReview 0</partinfo>
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<I>Username</I>
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<I>SCAU-China</I>
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Enter the review inofrmation here.
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The main goal of this characterization is to find out which pH value is the most effective among the 6 pH values in order to obtain a suitable culture condition for the expression of Dendar2.We further found that the part which expressed the protein Dendar2 worked well in E.coli strain DH10B, like in the DH5 alpha.
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Result and Findings
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1.pH 4.5 is found to be the best pH that is suitable for the expression of Dendar2 .
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2.Thered fluorescence from Dendar2 after photoconversion showed stronger fluorescent intensity than before.
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3.Compared with the  the expression pattern of Dendar2 as showed previously in DH5α, we concluded that the expression of Dendar2 works in both E.coli strains.
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Latest revision as of 06:15, 16 October 2018


User Reviews

SCAU-China

The main goal of this characterization is to find out which pH value is the most effective among the 6 pH values in order to obtain a suitable culture condition for the expression of Dendar2.We further found that the part which expressed the protein Dendar2 worked well in E.coli strain DH10B, like in the DH5 alpha.

Result and Findings

1.pH 4.5 is found to be the best pH that is suitable for the expression of Dendar2 .

2.Thered fluorescence from Dendar2 after photoconversion showed stronger fluorescent intensity than before.

3.Compared with the the expression pattern of Dendar2 as showed previously in DH5α, we concluded that the expression of Dendar2 works in both E.coli strains.

;