Difference between revisions of "Part:BBa K607003"
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<partinfo>BBa_K607003 short</partinfo> | <partinfo>BBa_K607003 short</partinfo> | ||
| − | GFP with LVA-tag under transcriptional control of a modified Prm(PcI) promoter | + | GFP with LVA-tag under transcriptional control of a modified Prm(PcI) promoter. |
| − | + | The Prm promotor is induced by cI. For more information on cI, see: https://parts.igem.org/wiki/index.php?title=Part:BBa_K607001<br> | |
| + | Activation of PRM involves an interaction between an acidic patch on the surface of the helix–turn–helix motif of cI protein and a target region near the C terminus of the σ70 subunit of Escherichia coli RNA polymerase. | ||
| + | The GFP protein contains an LVA tag, so it will be degraded faster by proteolytic enzymes compared to GFP proteins without LVA tag. | ||
| + | <br> | ||
| + | <br>References | ||
| + | <br> Changing the mechanism of transcriptional activation by phage λrepressor. Mei Li,* W. R. McClure,† and Miriam M. Susskind. Proc Natl Acad Sci U S A. 1997. | ||
| + | <br> | ||
| + | <br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 19:18, 21 September 2011
Prm_GFP-LVA
GFP with LVA-tag under transcriptional control of a modified Prm(PcI) promoter.
The Prm promotor is induced by cI. For more information on cI, see: https://parts.igem.org/wiki/index.php?title=Part:BBa_K607001
Activation of PRM involves an interaction between an acidic patch on the surface of the helix–turn–helix motif of cI protein and a target region near the C terminus of the σ70 subunit of Escherichia coli RNA polymerase.
The GFP protein contains an LVA tag, so it will be degraded faster by proteolytic enzymes compared to GFP proteins without LVA tag.
References
Changing the mechanism of transcriptional activation by phage λrepressor. Mei Li,* W. R. McClure,† and Miriam M. Susskind. Proc Natl Acad Sci U S A. 1997.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 752