Difference between revisions of "Part:BBa K678070:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K678070 short</partinfo>
 
<partinfo>BBa_K678070 short</partinfo>
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===Design Notes===
 
===Design Notes===
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To be announced
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This plasmid is not compatible with the Biobrick standards and is intended for [http://2011.igem.org/Team:DTU-Denmark-2/Project/introduction#USER%20cloning USER cloning].
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===Source===
 
===Source===
  
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To be announced
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'''Hansen, Bjarne G.; Bo Salomonsen; Morten T. Nielsen; Jakob B. Nielsen; Niels B. Hansen; Kristian F. Nielsen; Torsten B. Regueira; Jens Nielsen; Kiran R. Patil; and Uffe H. Mortensen'''; “Versatile enzyme expression and Characterization system for ''Aspergillus'', with the ''Penicillium brevicompactum'' Polyketide Synthase Gene from the Mycophenolic Acid Gene Cluster as a Test Case.” American Society for Microbiology, 2011, 3044-3051.
  
 
===References===
 
===References===

Latest revision as of 10:16, 20 September 2011

p68


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6337
    Illegal EcoRI site found at 11000
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 7126
    Illegal XbaI site found at 11325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6337
    Illegal EcoRI site found at 11000
    Illegal NotI site found at 1296
    Illegal NotI site found at 11282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6337
    Illegal EcoRI site found at 11000
    Illegal BglII site found at 3225
    Illegal BglII site found at 8299
    Illegal BglII site found at 10490
    Illegal BamHI site found at 6407
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6337
    Illegal EcoRI site found at 11000
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 7126
    Illegal XbaI site found at 11325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6337
    Illegal EcoRI site found at 11000
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 7126
    Illegal XbaI site found at 11325
    Illegal NgoMIV site found at 7043
    Illegal AgeI site found at 8847
    Illegal AgeI site found at 8877
    Illegal AgeI site found at 10254
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2122
    Illegal BsaI site found at 8680
    Illegal BsaI site found at 9852
    Illegal BsaI site found at 10446
    Illegal BsaI site found at 10642
    Illegal BsaI site found at 10813
    Illegal BsaI.rc site found at 3137
    Illegal BsaI.rc site found at 6757
    Illegal SapI.rc site found at 2793


Design Notes

This plasmid is not compatible with the Biobrick standards and is intended for [http://2011.igem.org/Team:DTU-Denmark-2/Project/introduction#USER%20cloning USER cloning].

Source

Hansen, Bjarne G.; Bo Salomonsen; Morten T. Nielsen; Jakob B. Nielsen; Niels B. Hansen; Kristian F. Nielsen; Torsten B. Regueira; Jens Nielsen; Kiran R. Patil; and Uffe H. Mortensen; “Versatile enzyme expression and Characterization system for Aspergillus, with the Penicillium brevicompactum Polyketide Synthase Gene from the Mycophenolic Acid Gene Cluster as a Test Case.” American Society for Microbiology, 2011, 3044-3051.

References