Difference between revisions of "Part:BBa K549003"

 
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<partinfo>BBa_K549003 short</partinfo>
 
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The biobrick contains the RcnA promotor from E. coli fused with the luxAB reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the Luciferase is expressed.
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The biobrick contains the RcnA promotor from E. coli fused with the ''luxAB'' reporter gene. ''E. coli'' strains own the RcnR-regulator gene which directly regulates the transcription of the RcnA nickel-cobalt efflux protein from which the promotor is amplified.
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In absence of Ni(II), RcnR represses the ''rcnA''-promoter.
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After addition of Ni (II) (NiCl2), RcnR binds this ion and is released from the promoter. Therefore, luxAB will be expressed.
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--> also use luxCDE or luciferine to detect output.
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In presence of nickel the Luciferase is expressed.
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The promoter is also a little bit sensitive for Co 2+.
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===Usage and Biology===
 
===Usage and Biology===
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This is how the BioBrick is working:
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[[Image:Bild-rcnaluxAB.png]]
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Latest revision as of 15:22, 30 September 2011

Nickel(II) dependent promotor with luxAB as reporter

The biobrick contains the RcnA promotor from E. coli fused with the luxAB reporter gene. E. coli strains own the RcnR-regulator gene which directly regulates the transcription of the RcnA nickel-cobalt efflux protein from which the promotor is amplified. In absence of Ni(II), RcnR represses the rcnA-promoter. After addition of Ni (II) (NiCl2), RcnR binds this ion and is released from the promoter. Therefore, luxAB will be expressed.

--> also use luxCDE or luciferine to detect output.

In presence of nickel the Luciferase is expressed.

The promoter is also a little bit sensitive for Co 2+.



Usage and Biology

This is how the BioBrick is working:

Bild-rcnaluxAB.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 751
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1270