Difference between revisions of "Part:BBa K549002"
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<partinfo>BBa_K549002 short</partinfo> | <partinfo>BBa_K549002 short</partinfo> | ||
The biobrick contains the RcnA promotor from E. coli fused with the lacZ' reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the LacZ' is expressed. | The biobrick contains the RcnA promotor from E. coli fused with the lacZ' reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the LacZ' is expressed. | ||
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Latest revision as of 21:27, 21 September 2011
Nickel(II) dependent promotor with lacZ' as reporter
The biobrick contains the RcnA promotor from E. coli fused with the lacZ' reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the LacZ' is expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]