Difference between revisions of "Part:BBa K678001:Design"

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===Design Notes===
 
===Design Notes===
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This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
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This part was designed for the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
  
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https://static.igem.org/mediawiki/igem.org/4/4d/Promotor_med_haler.png
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[[Image:Promotor_med_haler.png|left|250px|caption|<b>DTU-Denmark-2 2011</b>]]
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<br clear=all>
  
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===Source===
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This part can easily be fused with other parts adapted to the Plug 'n' Play assembly standard as shown below.
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''Aspergillus nidulans'' genomic DNA
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===References===
 
  
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(1) '''Lubertozzi, D., and Keasling, J.D.''': Marker and promoter effects on heterologous expression in ''Aspergillus nidulans''. Appl Microbiol Biotechnol (2006) 72: 1014–1023.
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[[Image:Overveiw_module_tails_2.png|left|450px|caption|<b>DTU-Denmark-2 2011</b>]]
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<br clear=all>
  
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(2) '''Creaser, E.H., Porter, R.L., Britt, K.A., Pateman, J.A. and, Doy, C.H.''': Purification and preliminary characterization of alcohol dehydrogenase from ''Aspergillus nidulans''. Biochem. J. (1985) 225: 449-454.
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This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug 'n' Play plasmids [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678058 pJEJAM10], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678059 pJEJAM11], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678067 pJEJAM19], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678068 pJEJAM20]
  
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(3) '''Bailey, C., and Arst Jr., H.N.''': Carbon catabolite repression in ''Aspergihs nidulans''. Eur. J. Biochem. (1975) 51: 573-577.
 
  
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(4) '''Waring, R.B., May, G.S., and Morris, N.R.''': Characterization of an inducible expression system in ''Aspergillus nidulans'' using ''alcA'' and tuhulin- coding genes.  Gene, (1989) 79: 119-130.
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===Source===
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''Aspergillus nidulans'' genomic DNA
 +
 
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===References===
  
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(5) '''Romero, B., Turner, G., Olivas, I., Laborda, F., and De Lucas, J.R.''': The ''Aspergillus nidulans alcA'' promoter drives tightly regulated conditional gene expression in ''Aspergillus fumigatus'' permitting validation of essential genes in this human pathogen. Fungal Genetics and Biology (2003) 40: 103–114.
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'''Creaser, E.H., Porter, R.L., Britt, K.A., Pateman, J.A. and, Doy, C.H.''': Purification and preliminary characterization of alcohol dehydrogenase from ''Aspergillus nidulans''. Biochem. J. (1985) 225: 449-454.

Latest revision as of 08:05, 27 September 2011

PalcA, inducible Aspergillus nidulans promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.

DTU-Denmark-2 2011


This part can easily be fused with other parts adapted to the Plug 'n' Play assembly standard as shown below.


DTU-Denmark-2 2011


This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug 'n' Play plasmids pJEJAM10, pJEJAM11, pJEJAM19, and pJEJAM20


Source

Aspergillus nidulans genomic DNA

References

Creaser, E.H., Porter, R.L., Britt, K.A., Pateman, J.A. and, Doy, C.H.: Purification and preliminary characterization of alcohol dehydrogenase from Aspergillus nidulans. Biochem. J. (1985) 225: 449-454.