Difference between revisions of "Part:BBa K537003"

 
 
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<partinfo>BBa_K537003 short</partinfo>
 
<partinfo>BBa_K537003 short</partinfo>
  
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This is a theohylline riboswitch clone 8.1 (Topp and Gallivan JACS 2007) also BBa_K249026 and BBa_K411001 fusion with Venus reporter BBa_K354002. This part regulates the expression of venus in a theophylline-dependent fashion through an RNA aptmer specific to theophylline.   
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This part regulates the expression of venus in a theophylline-dependent fashion through an RNA aptamer specific to theophylline.   
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This theophylline riboswitch (type1) biobrick activates the expression of the Venus fluorescent protein in a theophylline-dependent fashion. It is composed of a theophylline sensitive riboswitch clone 8.1 (Topp and Gallivan JACS 2007; BBa_K249026 and BBa_K411001) fused upstream to an adjacent Venus coding region.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K537003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K537003 SequenceAndFeatures</partinfo>
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<p><big>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/parts/2/27/Datasheet1.pdf">here</a></big></p>
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<center><img src="https://static.igem.org/mediawiki/parts/5/5b/Datasheet1.jpg" width="910" vspace="15"></center>
  
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K537003 parameters</partinfo>
 
<partinfo>BBa_K537003 parameters</partinfo>
 
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Latest revision as of 19:35, 28 October 2011

Theophylline Riboswitch 1-Venus

This part regulates the expression of venus in a theophylline-dependent fashion through an RNA aptamer specific to theophylline.

This theophylline riboswitch (type1) biobrick activates the expression of the Venus fluorescent protein in a theophylline-dependent fashion. It is composed of a theophylline sensitive riboswitch clone 8.1 (Topp and Gallivan JACS 2007; BBa_K249026 and BBa_K411001) fused upstream to an adjacent Venus coding region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



For the pdf version of this datasheet, click here