Difference between revisions of "Part:BBa K538200:Design"

Latest revision as of 19:03, 19 September 2011

pLacI + RBS + Cpn10

LacI:Cpn10 K538200

This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into E. coli, it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (pLacI: BBa_R0010) so that it may be expressed constitutively in LacI-null strains, or inducibly in LacI-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as BBa_K538000.

For more information about the subparts' purpose and design, refer to their main page or design page, respectively.

BBa_R0010
- Main Page
- Design Page
BBa_B0034
- Main Page
- Design Page
BBa_K538000
- Main Page
- Design Page

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 278

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K538200 short</partinfo>
-<partinfo>BBa_K538200 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K538200 Icon</partinfo>
-===Design Notes===
+This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into ''E. coli'', it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (''pLacI'': [[Part:BBa_R0010|BBa_R0010]]) so that it may be expressed constitutively in ''LacI''-null strains, or inducibly in ''LacI''-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as [[Part:BBa_K538000|BBa_K538000]].
--
+For more information about the subparts' purpose and design, refer to their main page or design page, respectively.
+* '''BBa_R0010'''
+** [[Part:BBa_R0010|Main Page]]
+** [[Part:BBa_R0010:Design|Design Page]]
+* '''BBa_B0034'''
+** [[Part:BBa_B0034|Main Page]]
+** [[Part:BBa_B0034:Design|Design Page]]
+* '''BBa_K538000'''
+** [[Part:BBa_K538000|Main Page]]
+** [[Part:BBa_K538000:Design|Design Page]]
-===Source===
+<partinfo>BBa_K538200 SequenceAndFeatures</partinfo>
--
-===References===