Difference between revisions of "Part:BBa K538130"

 
 
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<partinfo>BBa_K538130 short</partinfo>
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<partinfo>K538130 short</partinfo>
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<partinfo>K538130 Icon</partinfo>
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This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: '''''icE. coli'''''. It was used as a construction intermediate, to create the following composite parts:
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* [[Part:BBa_K538300 | BBa_K538300]]
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* [[Part:BBa_K538301 | BBa_K538301]]
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* [[Part:BBa_K538302 | BBa_K538302]]
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* [[Part:BBa_K538303 | BBa_K538303]]
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* [[Part:BBa_K538304 | BBa_K538304]]
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* [[Part:BBa_K538310 | BBa_K538310]]
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* [[Part:BBa_K538311 | BBa_K538311]]
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Note this part contains no Bricks that are new to the registry; its subparts, [[Part:BBa_K206000 | BBa_K206000]] and [[Part:BBa_B0034 | BBa_B0034]], were part of 2011's Spring Distribution kit. Team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] used it to create the various protein generators listed above, by appending one of the parts below:
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* [[Part:BBa_K538000 | BBa_K538000]]: Cpn10 (from ''Oleispira antarctica'')
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* [[Part:BBa_K538001 | BBa_K538001]]: Cpn60 (from ''Oleispira antarctica'')
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* [[Part:BBa_K538002 | BBa_K538002]]: SheDnak (from ''Shewanella'' sp. AC10)
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* [[Part:BBa_K538003 | BBa_K538003]]: CspA (from ''Polaribacter irgensii'')
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* [[Part:BBa_K538004 | BBa_K538004]]: CspC (from ''Polaribacter irgensii'')
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In addition to this, for the creation of [[Part:BBa_K538310 | BBa_K538310]] and [[Part:BBa_K538211 | BBa_K538211]], one of these parts was also appended:
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* [[Part:BBa_K538100 | BBa_K538100]]: RBS + Cpn10
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* [[Part:BBa_K538101 | BBa_K538101]]: RBS + Cpn60
  
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===Usage and Biology===
 
  
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These bricks all encode a protein that should confer a degree of cold resistance when expressed by ''E. coli''. The biology behind these cold resistance BioBricks, or CryoBricks, is explained in greater detail on the pages of the individual coding regions.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K538130 SequenceAndFeatures</partinfo>
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===Sequence and Features===
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===Functional Parameters===
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<partinfo>K538130 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K538130 parameters</partinfo>
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Latest revision as of 12:29, 19 September 2011

pBAD + RBS


K538130


This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. It was used as a construction intermediate, to create the following composite parts:


Note this part contains no Bricks that are new to the registry; its subparts, BBa_K206000 and BBa_B0034, were part of 2011's Spring Distribution kit. Team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] used it to create the various protein generators listed above, by appending one of the parts below:

In addition to this, for the creation of BBa_K538310 and BBa_K538211, one of these parts was also appended:


These bricks all encode a protein that should confer a degree of cold resistance when expressed by E. coli. The biology behind these cold resistance BioBricks, or CryoBricks, is explained in greater detail on the pages of the individual coding regions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]