Difference between revisions of "Part:BBa K515010"

 
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<partinfo>BBa_K515010 short</partinfo>
 
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pVeg with one substitution mutation
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<p>After sequencing part <a href="https://parts.igem.org/Part:BBa_K316005:Design">BBa_K316005</a> during the assembly process of <a href="https://parts.igem.org/Part:BBa_K515100:Design">BBa_K515100</a> we found that there was a one base pair mutation in the promoter. We named this new version of the promoter pVeg2 and used it in our construct.</p>
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<partinfo>BBa_K515010 parameters</partinfo>
 
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<p><b>This BioBrick has been sequence verified.</p></b>
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<p>We have made a protein gel in order to verify whether the promoter is over-expressing XylE in the <a href="https://parts.igem.org/Part:BBa_K316005">BBa_K316005</a> construct in a DH5-α strain of <i>Escherichia coli</i>. The results showed a clear thick band corresponding to the correct size of the protein.</p>
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<img class="border" style="border-color:#B2B2B2;" src="https://static.igem.org/mediawiki/parts/0/02/ICL_XylE_pVeg2_Protein_Gel.png" width=600px/>
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<p><i>Protein gel of XylE and control cells. Lane 1 contains the XylE producing <i>E. coli</i> and lane two contains the control cell line. The Ladder is a Fermentas PageRuler.</i></p>
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Latest revision as of 16:17, 10 May 2013

pVeg2

After sequencing part BBa_K316005 during the assembly process of BBa_K515100 we found that there was a one base pair mutation in the promoter. We named this new version of the promoter pVeg2 and used it in our construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

We have made a protein gel in order to verify whether the promoter is over-expressing XylE in the BBa_K316005 construct in a DH5-α strain of Escherichia coli. The results showed a clear thick band corresponding to the correct size of the protein.

Protein gel of XylE and control cells. Lane 1 contains the XylE producing E. coli and lane two contains the control cell line. The Ladder is a Fermentas PageRuler.