Difference between revisions of "Part:BBa K515100:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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===Applications of BBa_K515100===
 
===Applications of BBa_K515100===
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*The enzymatic reactions of the IAM pathway produce indole-3 acetic acid (IAA) which is an important phytohormone.
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*Many Plant Growth Promoting Rhizobacteria produce IAA
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*Analogues of phytohormones like α-Naphthaleneacetic acid are usually found in rooting powders. Therefore, if we were to express IAA in bacteria in a controlled fashion we could increase root growth.
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*Increased root growth could help maintain top soil.
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*Evidence has shown that using IAA on cotton can increase yields.
  
 
===User Reviews===
 
===User Reviews===
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{|width='80%' style='border:1px solid gray'
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|-
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|width='10%'|
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<partinfo>BBa_K515100 AddReview 5</partinfo>
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<I>NUDT_CHINA 2015</I>
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|width='60%' valign='top'|
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This part is the only one available with IAAM and IAAH , but unfortunately, we noticed that neither of the CDS of those two enzyme were submitted to the registry (they were only registered as BBa_K515000 and K515001). Thus, several improvements were made based on the part K515100 to provide an available version of IAAM and IAAH.
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====Two pairs of enzymes that can PCR-prep the CDS of IaaM and IaaH====
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For IAAM
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F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’
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R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’
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For IAAH
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F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’
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R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’
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====Two new bio-bricks were designed based on this part using the primers above====
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See BBa_K1789000 and BBa_K1789001
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Both of those parts were sent to the registry.
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<!-- DON'T DELETE --><partinfo>BBa_K515100 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K515100 EndReviews</partinfo>

Latest revision as of 16:27, 18 September 2015

Applications of BBa_K515100

  • The enzymatic reactions of the IAM pathway produce indole-3 acetic acid (IAA) which is an important phytohormone.
  • Many Plant Growth Promoting Rhizobacteria produce IAA
  • Analogues of phytohormones like α-Naphthaleneacetic acid are usually found in rooting powders. Therefore, if we were to express IAA in bacteria in a controlled fashion we could increase root growth.
  • Increased root growth could help maintain top soil.
  • Evidence has shown that using IAA on cotton can increase yields.

User Reviews

UNIQ6b421f18c0b4cb19-partinfo-00000001-QINU


•••••

NUDT_CHINA 2015

This part is the only one available with IAAM and IAAH , but unfortunately, we noticed that neither of the CDS of those two enzyme were submitted to the registry (they were only registered as BBa_K515000 and K515001). Thus, several improvements were made based on the part K515100 to provide an available version of IAAM and IAAH.

Two pairs of enzymes that can PCR-prep the CDS of IaaM and IaaH

For IAAM

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’

R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’


For IAAH

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’

R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’

Two new bio-bricks were designed based on this part using the primers above

See BBa_K1789000 and BBa_K1789001

Both of those parts were sent to the registry.

;


UNIQ6b421f18c0b4cb19-partinfo-00000003-QINU