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| − | __NOTOC__
| + | #redirect [[Help:Submission]] |
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| − | ===2011 Submission Details===
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| − | <span style="color:#148800; font-weight:bold; font-size:120%;"> All parts must be submitted in <partinfo>pSB1C3</partinfo>.</span> The Registry only accepts parts in the standard DNA-shipping plasmid backbone: <partinfo>pSB1C3</partinfo>. The 2011 DNA Distribution kits contained samples of linearized plasmid backbones for easy use. You can also [[Help:Protocols/Linearized_Plasmid_Backbones | make your own]] linearized plasmid backbone or request more by sending an email to '''hq [at] igem [dot] org'''.
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| − | <p>
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| − | <span style="color:#148800; font-weight:bold; font-size:120%;">"Quality over quantity"</span> is our motto this year! Teams and labs should only submit parts that are well documented and characterized. For examples of well documented parts check out <partinfo>BBa_K274210</partinfo>, <partinfo>BBa_F2620</partinfo>, and <partinfo>BBa_E0040</partinfo>.
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| − | ===Starting a New DNA Submission===
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| − | <span style="color:#148800; font-weight:bold; font-size:120%;">Before starting a submission make sure that all the parts you intend to send are documented on the Registry. [https://parts.igem.org/wiki/index.php/Add_a_Part_to_the_Registry Add a part to the Registry]</span>
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| − | When you are ready to send your parts to the Registry, go to the [https://parts.igem.org/cgi/dna_transfer/index.cgi DNA Submission main page]. You will be able to “start a new DNA submission” or “see your DNA submissions now.” To begin the submission process click on '''“start a new DNA submission.”'''
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| − | [[Image:DNA_Submissions__main_page_options.png|400px|center]]
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| − | <br style="clear:both;"/>
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| − | ====Information====
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| − | On this page that you must enter information about yourself as the user submitting the DNA. Please make sure that the username and full name that we have listed for you is correct. You then need to choose which team or lab you are part of (also called a group). Correct your email if necessary and do not forget to enter your phone number. [[Image:Contact_info_check.png|500px|center]]
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| − | <br style="clear:both;"/>
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| − | ====Format====
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| − | You must tell us in what format you are sending your DNA. Please be sure to follow [[DNA Submission Formats|'''the preparation requirements''']] for the accepted formats. You should also let us know if there are any special instructions that we must know about.
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| − | [[Image:Formats.png|300px|center]]
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| − | <br style="clear:both;"/>
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| − | Proceed to entering sample information by clicking on the button at the bottom of the page. [[Image:Proceed_to_sample_info.png|350px|center]]
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| − | ====Sample Information====
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| − | At the top of the sample information page you will see your Shipment #, which is what we use to identify and keep track of your shipment. <span style="color:#148800; font-weight:bold; font-size:120%;">The shipment # must be written clearly on your submission.</span>
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| − | [[Image:Sample_details.png|600px|center]]
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| − | Enter the information for your first part, beginning with the part number. You must have your part documented on the Registry before you can add it as a sample. You must enter the plasmid backbone that is paired with the part <span style="color:#148800; font-weight:bold; font-size:120%;">(<partinfo>pSB1C3</partinfo>)</span>. See the “Notes” section below for more details on plasmid information.
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| − | Once you have entered the part number and plasmid for your part, you can tab over to the antibiotic resistance field. The software will automatically fill in the antibiotic resistance based on the plasmid that you have entered. Please check to make sure that the resistance is correct. You may change it manually if it is not correct. The antibiotic abbreviation is the first letter of the antibiotic.
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| − | '''Please check any of the boxes that apply to your part if:'''
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| − | #It is toxic to E.coli strains
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| − | #You are only sending a plasmid
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| − | #It is not a part or plasmid
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| − | #Your team has sequenced this part
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| − | We need to know specifically whether your part is toxic to the '''NEB 10b''' strain. We will be transforming all parts into NEB 10b and we need to know in advance whether this will be a problem with any of your parts.
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| − | If you are sending us a plasmid, you need to check the appropriate box (see below for more plasmid information).
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| − | Please let us know by checking the box if you or your team has sequenced this part. This is '''not''' a box to have your part sequenced. At the moment we will not be collecting any sequence data or trace files but at some point in the future we will enable this feature. Please save all of your sequencing data in the event that you would like to add that information at a later date.
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| − | At this point you may add any notes that you may want to communicate with the Registry regarding your part in the comments field.
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| − | [[Image:Sample_info_2.png|500px|center]]
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| − | Once you have finished adding all of the information for your part, <font color=red>'''click on the “save” link'''</font> and then “add another sample” button if you are sending more parts to the Registry. The information for each sample is automatically saved to this shipment number once you click on the “save” link.
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| − | ====Shipment Information====
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| − | When you are done filling in the information for all of your samples, click on the “I am finished specifying the samples” button. [[Image:Finished_specifying_samples.png|300px]] <u>Be aware that your shipment has not yet been finalized (even though it has been saved)</u>. To do so, you must fill in all of your shipping information. Please provide ALL information.
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| − | [[Image:Shipping_details.png|350px|center]]
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| − | <span style="color:#148800; font-weight:bold; font-size:120%;">Be prepared with your shipping details!</span> Once you select '''"I am finished filling in the information, Send this batch to the Registry"''' you will no longer be able to edit your shipment as it will be finalized. We recommend having all your details (Carrier, Tracking #, and Send Date) on hand as you enter the information. If you have finalized your shipment but did not add a tracking number you can send an email to '''hq [at] igem [dot] org''' with your team name, shipment #, and tracking #.
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| − | When you are finished providing shipping details, click on the “I am finished filling in this information” button to send your information to the Registry.[[Image:Send_to_Registry.png|100px]]
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| − | This finalizes your shipment information. You will see a confirmation page that congratulates you on submitting your information. It will also show all parts that are part of that shipment number. We recommend printing out this page for your records.
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| − | At this point you can go back to the DNA Submission Registry page and click on “see your DNA submissions” to check on the status of your shipment. You will be able to see when we have received your shipment and once we have finished testing your parts, you will be able to see the results from our quality control tests.
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| − | <!--
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| − | If for any reason we reject your part we will notify you by email (see below for possible reasons your part might be rejected). The email will be sent to the user who filled out the online submission form. The rest of the parts in your shipment that passed the quality control tests will be marked as Accepted but you will have to resubmit the parts that did not pass successfully. These parts must be resubmitted as a new shipment using the online DNA submission form once again.
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| − | Reasons for failing quality control:
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| − | * Failed to transform
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| − | * Did not grow in culture
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| − | * Growth in wrong antibiotic broth in antibiotic test
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| − | * Wrong fragment size when cut with double restriction enzymes
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| − | -->
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| − | ====Shipping Instructions====
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| − | <span style="color:#148800; font-weight:bold; font-size:120%;">Make sure your team name, shipment #, and send date are written clearly on your submission!</span>
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| − | We also highly recommend using a shipping carrier that that will let you track the shipment. For iGEM competition teams, physical DNA for parts is due before their Regional Jamboree and if we do not receive your shipment, we will track your package and give that information to the judges. If your shipment does not have tracking information and has not arrived at the Registry we will count your physical DNA as '''not available'''. You will not be able to receive a medal, as per the iGEM requirements.
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| − | '''<span style="color:red;">*Important*'''</span>: When shipping your samples, make sure to include a detailed description of the package so that it gets through customs. Write the following:<br>
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| − | <span style="color:red; font-weight:bold; margin-left:200px;">Dry/liquid DNA, non-hazardous, non-infectious, non-regulated. For research use only.</span>
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| − | ===Notes===
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| − | * '''Accepting bacteria'''
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| − | ** The Registry does not accept bacteria shipments at this time. Please only send isolated plasmid DNA. You may contact us if you have any questions regarding this policy.
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| − | * '''Editing your shipment after it has been finalized'''
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| − | ** You may edit your shipment at any point up until the time you finalize your shipment after entering your shipping details. After you have finalized your shipment and sent all parts and information to the Registry, you will not be able to edit your online submission form. If you need to make a change to your submission form information, you must contact hq [at] igem [dot] org. Please include your shipment number, your name, your team name, and the sample information that you would like to change.
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| − | * '''Part and plasmid specification details'''
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| − | ** In specifying your parts you must clearly understand the relationship between parts and plasmids. You must be explicitly aware that a part does NOT include the flanking restriction enzyme sites (called BioBrick™ [[Help:BioBrick_Prefix_and_Suffix | prefix and suffix]] if you are using the BioBrick™ standard for which the restriction enzymes are: EcoRI, XbaI, SpeI, PstI). These sites are included in the plasmid backbone that your part is in (pSB1C3). Your part, then, begins with the first letter (A, C, T, G) following the prefix cloning site of the plasmid and ends with the last letter preceding the suffix cloning site of the plasmid.
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| − | ** Take a look at [[Help:An Introduction to BioBricks|'''this help page''']] for more information on Biobrick™ parts and plasmids and how they are defined.
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| − | <font color=mediumblue>'''Useful links:'''</font>
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| − | * [[Help:An_Introduction_to_BioBricks|Introduction to Biobricks™]]: including details on part and plasmid definitions.
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| − | <font color=cornflowerblue>'''Have any questions? Contact hq [AT] igem [DOT] org.'''</font>
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