Difference between revisions of "Part:BBa K549001"

 
 
(17 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K549001 short</partinfo>
 
<partinfo>BBa_K549001 short</partinfo>
  
−
E. coli has an RcnR-regulator that switches on the RcnA-Promotor in the presence of Ni(II). After induction GFP will be expressed.
+
E. coli has an RcnR-regulator that represses the ''rcnA''-promoter in the absence of Ni(II). After addition of Ni (II) (NiCl2), RcnR binds this ion and is released from the promoter. Therefore, GFP will be expressed.
 +
 
 +
E. coli has to be used as reporter organism since RcnR is not coded on the plasmid.
 +
 
 +
In presence of nickel the Luciferase is expressed.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
 +
This BioBrick can be used to detect Ni(II)-Ions in water.
 +
 +
This is how it works:
 +
 +
[[Image:Bild-rcnAGFP.png]]
 +
 +
The sensor is also sensing Co 2+, but not very sensitivly.
 +
 +
 +
Sources:
 +
- [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05369.x/pdf Nickel homeostasis in Escherichia coli – the rcnR-rcnA efflux pathway and its linkage to NikR function] Iwig JS, Rowe JL, Chivers PT <br>
 +
- [http://www.ncbi.nlm.nih.gov/pubmed?term=coordinating%20intracellular%20chivers Coordinating intracellular nickel-metal-site structure-function relationships and the NikR and RcnR repressors] Iwig JS, Chivers PT<br>
 +
 +
<!-- Add more about the biology of this part here
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 17:34, 30 September 2011

Ni(II) dependent rcnA-promoter and GFP

E. coli has an RcnR-regulator that represses the rcnA-promoter in the absence of Ni(II). After addition of Ni (II) (NiCl2), RcnR binds this ion and is released from the promoter. Therefore, GFP will be expressed.

E. coli has to be used as reporter organism since RcnR is not coded on the plasmid.

In presence of nickel the Luciferase is expressed.

Usage and Biology

This BioBrick can be used to detect Ni(II)-Ions in water.

This is how it works:

Bild-rcnAGFP.png

The sensor is also sensing Co 2+, but not very sensitivly.


Sources: - [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05369.x/pdf Nickel homeostasis in Escherichia coli – the rcnR-rcnA efflux pathway and its linkage to NikR function] Iwig JS, Rowe JL, Chivers PT
- [http://www.ncbi.nlm.nih.gov/pubmed?term=coordinating%20intracellular%20chivers Coordinating intracellular nickel-metal-site structure-function relationships and the NikR and RcnR repressors] Iwig JS, Chivers PT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863