Difference between revisions of "Part:BBa K091104:Experience"

(→‎User Reviews)
(→‎User Reviews)
 
(7 intermediate revisions by one other user not shown)
Line 20: Line 20:
 
|-
 
|-
 
|width='10%'|
 
|width='10%'|
−
<partinfo>BBa_K091104 AddReview number</partinfo>
+
<partinfo>BBa_K091104 AddReview 1</partinfo>
−
<I>SarahV</I>
+
<I><B>[http://2011.igem.org/Team:KULeuven K.U.Leuven iGEM 2011 Team]</B></I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI.  Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp.  
+
<u><b>Characterization by K.U.Leuven 2011 iGEM Team</b></u>
−
However, we see a fragment of 1000bp and of ±2000bp.  
+
 
 +
We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI.  Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp.  
 +
However, we see a fragment of 1000bp and one of ±2000bp.  
 +
[[Image:Restriction_1807.jpg|thumb|center|360px|]]
 
|}
 
|}
−
[[Image:Restriction_1807.jpg]]
+
 
  
 
<!-- DON'T DELETE --><partinfo>BBa_K091104 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K091104 EndReviews</partinfo>

Latest revision as of 08:52, 27 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K091104

User Reviews

UNIQde22a77078f6a8be-partinfo-00000000-QINU

•

[http://2011.igem.org/Team:KULeuven K.U.Leuven iGEM 2011 Team]

Characterization by K.U.Leuven 2011 iGEM Team

We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and one of ±2000bp.

Restriction 1807.jpg


UNIQde22a77078f6a8be-partinfo-00000002-QINU