Difference between revisions of "Part:BBa K523007"

Latest revision as of 20:52, 16 September 2011

Plac + LacZ + malS (dubious version)

E. coli periplasmic alpha-amylase gene malS under control of lac promoter. LacZ is also present for blue/white screening.

This contains an artificial RBS, and no stop codon... furthermore the 2 extra G bases at the end of the malS sequence (see BBa_K523003) mean there's not even a stop codon provided by the BioBrick suffix, since it's now in a different frame.

This part might therefore not be of any use to anyone, even ourselves...

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1830
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 625
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 966
Illegal AgeI site found at 2131
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 ''E. coli'' periplasmic alpha-amylase gene ''malS'' under control of lac promoter. LacZ is also present for blue/white screening.
-This contains an artificial RBS, and no stop codon...
+This contains an artificial RBS, and no stop codon... furthermore the 2 extra G bases at the end of the malS sequence (see <partinfo>BBa_K523003</partinfo>) mean there's not even a stop codon provided by the BioBrick suffix, since it's now in a different frame.
+This part might therefore not be of any use to anyone, even ourselves...
 <!-- Add more about the biology of this part here
@@ Line 10: / Line 13: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'>'''Sequence and Features'''</span>
 <partinfo>BBa_K523007 SequenceAndFeatures</partinfo>