Difference between revisions of "Part:BBa K523005"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K523005 short</partinfo> | <partinfo>BBa_K523005 short</partinfo> | ||
− | LacZ reporter followed by | + | Plac promoter (with RBS) followed by LacZ reporter followed by another RBS. |
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | This is intended to be used as a promoter and RBS; i.e. it can be added upstream of a coding sequence to cause that sequence to become expressed. | |
− | <span class='h3bb'>Sequence and Features</span> | + | |
+ | The LacZ reporter will cause lab strains with the correct genotype to turn blue in the presence of Xgal+IPTG. | ||
+ | |||
+ | <span class='h3bb'>'''Sequence and Features'''</span> | ||
<partinfo>BBa_K523005 SequenceAndFeatures</partinfo> | <partinfo>BBa_K523005 SequenceAndFeatures</partinfo> | ||
Latest revision as of 20:36, 16 September 2011
Plac-RBS-LacZ-RBS
Plac promoter (with RBS) followed by LacZ reporter followed by another RBS.
Usage and Biology
This is intended to be used as a promoter and RBS; i.e. it can be added upstream of a coding sequence to cause that sequence to become expressed.
The LacZ reporter will cause lab strains with the correct genotype to turn blue in the presence of Xgal+IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]