Difference between revisions of "Part:BBa I13521:Design"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I13521 short</partinfo>
 
<partinfo>BBa_I13521 short</partinfo>
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===Design Notes===
 
===Design Notes===
  
 +
Primers:
 +
Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix.
 +
Sequences provided by Gingko BioWorks.
  
 +
Fwd: GCTTCTAGAGATACATGAACATGCAATACtccctatcagtgatagagattgacatcc
  
 +
Rv: CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg
  
 
===Source===
 
===Source===
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===References===
 
===References===
 +
 +
<h2>The improvements submitted by iGEM17_SCU-China</h2>
 +
https://parts.igem.org/Part:BBa_K2276007
 +
https://parts.igem.org/Part:BBa_K2276010

Latest revision as of 07:36, 31 October 2017

Ptet mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.

Fwd: GCTTCTAGAGATACATGAACATGCAATACtccctatcagtgatagagattgacatcc

Rv: CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg

Source

References

The improvements submitted by iGEM17_SCU-China

https://parts.igem.org/Part:BBa_K2276007 https://parts.igem.org/Part:BBa_K2276010