Difference between revisions of "Part:BBa K523000:Design"

(→‎Source)
(→‎Source)
 
(2 intermediate revisions by the same user not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Note that the BglII (agatct) site overlaps the BioBrick prefix. Since the prefix ends "ag", only "atct" is included in the BioBrick proper.
 
  
 +
Made with PCR from <partinfo>BBa_J33207</partinfo> using primers:
  
 +
* '''Forward:''' gcttctagagatctgctggggcaaaccagcg
 +
* '''Reverse:''' a standard reverse primer for amplifying BioBricks
  
 
===Source===
 
===Source===
  
−
Based on part <partinfo>BBa_J33207</partinfo>, which was amplified from ''E. coli'' BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).
+
Part <partinfo>BBa_J33207</partinfo> was originally amplified from ''E. coli'' BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).
  
 
===References===
 
===References===

Latest revision as of 16:03, 7 September 2011

Plac + lacZ; with BglII site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made with PCR from BBa_J33207 using primers:

  • Forward: gcttctagagatctgctggggcaaaccagcg
  • Reverse: a standard reverse primer for amplifying BioBricks

Source

Part BBa_J33207 was originally amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).

References