Difference between revisions of "Part:BBa M36702"

 
 
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<partinfo>BBa_M36702 short</partinfo>
 
<partinfo>BBa_M36702 short</partinfo>
  
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Similar to BBa_M36701, this is a coupling of the Pyruvate sensor (BBa_M36698) with the Gemini Protein Generator (BBa_M36709)
  
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IclR binds with pyruvate to form a more stable structure to bind to the inhibitory regulation sites on the aceBAK promoter. By combining the IclR gene and the aceBAK promoter into the same plasmid, the presence of IclR and pyruvate will inhibit the aceBAK promoter and any genes downstream of it, in this case Gemini.  While there will be some residual transcription in the presence of only IclR, the presence of pyruvate increases the stability of binding with the aceBAK promoter region so inhibitory activities are higher. We choose to order to pieces of DNA to test the influence of signal leveling between the frequency of the IclR gene and aceBAK promoter. In this composite, the IclR gene is occurring in an equal frequency as the aceBAK promoter. See the Part Design for more information accounting for the ability of IclR to inhibit the aceBAK promoter.
  
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Similar to BBa_M36701, this is a coupling of the Pyruvate sensor (BBa_M36698) with the Gemini Protein Generator (BBa_M36709)
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:38, 6 May 2011

Pyruvate Sensor v2 w/ Gemini

Similar to BBa_M36701, this is a coupling of the Pyruvate sensor (BBa_M36698) with the Gemini Protein Generator (BBa_M36709)

IclR binds with pyruvate to form a more stable structure to bind to the inhibitory regulation sites on the aceBAK promoter. By combining the IclR gene and the aceBAK promoter into the same plasmid, the presence of IclR and pyruvate will inhibit the aceBAK promoter and any genes downstream of it, in this case Gemini. While there will be some residual transcription in the presence of only IclR, the presence of pyruvate increases the stability of binding with the aceBAK promoter region so inhibitory activities are higher. We choose to order to pieces of DNA to test the influence of signal leveling between the frequency of the IclR gene and aceBAK promoter. In this composite, the IclR gene is occurring in an equal frequency as the aceBAK promoter. See the Part Design for more information accounting for the ability of IclR to inhibit the aceBAK promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 679
    Illegal BamHI site found at 1196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2106