Difference between revisions of "Part:BBa M36699"
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<partinfo>BBa_M36699 short</partinfo> | <partinfo>BBa_M36699 short</partinfo> | ||
| − | A portion of the aceBAK promoter region that comprises the promoter as well as the IclR binding sites. | + | A portion of the aceBAK promoter region that comprises of the promoter as well as the IclR binding sites. |
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| + | IclR inhibits the aceBAK gene in conjunction with pyruvate or glyoxylate by binding these sites on the aceBAK promoter. There are 8 IclR binding sites in our version of the aceBAK promoter. When occupied, these binding sites inhibit the ability of the polymerase to bind to the DNA. Thus the more likely the IclR is to bind the aceBAK promoter, the less likely it is to have downstream genes expressed. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 21:03, 6 May 2011
PaceBAK
A portion of the aceBAK promoter region that comprises of the promoter as well as the IclR binding sites.
IclR inhibits the aceBAK gene in conjunction with pyruvate or glyoxylate by binding these sites on the aceBAK promoter. There are 8 IclR binding sites in our version of the aceBAK promoter. When occupied, these binding sites inhibit the ability of the polymerase to bind to the DNA. Thus the more likely the IclR is to bind the aceBAK promoter, the less likely it is to have downstream genes expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 207
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]