Difference between revisions of "Part:BBa J70638:Design"

(→‎Source)
(→‎Source)
 
Line 14: Line 14:
 
derived from K274002  
 
derived from K274002  
  
−
Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC
+
J70638 A Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC
  
−
Suffix: GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGATCCTTAGGCCTCGCGGCTCAG
+
J70638 B Suffix: GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGATCCTTAGGCCTCGCGGCTCAG
  
 
===References===
 
===References===

Latest revision as of 06:44, 19 November 2010

rbs VioA rbs VioB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal AgeI site found at 4010
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

there was a sequence between the stop codon of vioB and rbs of vioC in original part


Source

derived from K274002

J70638 A Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC

J70638 B Suffix: GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGATCCTTAGGCCTCGCGGCTCAG

References