Difference between revisions of "Part:BBa K498000:Experience"

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Both T9002 and K498000 HSL-detector devices were ligated into one of three different plasmids during the course of our work.  T9002 it its original plasmid was used to construct K498000 by incorporating the I172017 (Lac Z) part.  Both original T9002 and the composite part K498000 were used to successfully transform Agrobacteria by electroporation (Micro Pulser Electroporator, Bio-Rad protocol).  The composite part K498000 was also reconstructed in both pSB1AT3 and pSB1C1 independently through the A3 assembly method.  Agrobacteria was successfully transformed with all of the constructs.  Since chloramphenicol and tetracycline are protein synthesis inhibitors, all HSL-stimulation experiments were done in pSB1AT3 with ampicillin selection.
 
 
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Both T9002 and K498000 HSL-detector devices were ligated into one of three different plasmids during the course of our work.  T9002 it its original plasmid was used to construct K498000 by incorporating the I172017 (Lac Z) part.  Both original T9002 and the composite part K498000 were used to successfully transform Agrobacteria by electroporation (Micro Pulser Electroporator, Bio-Rad protocol).  The composite part K498000 was also reconstructed in both pSB1AT3 and pSB1C1 independently through the A3 assembly method.  Agrobacteria was successfully transformed with all of the constructs.  Since chloramphenicol and tetracycline are protein synthesis inhibitors, all HSL-stimulation experiments were done in pSB1AT3 with ampicillin selection.
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https://static.igem.org/mediawiki/parts/2/21/IvyTech-South_Bend_Experience.png

Latest revision as of 20:08, 7 November 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

We successfully assembled our new hybrid part BBa_K498000and transformed E. coli Top 10 cells yet have yet to get the reporter fluorsence and beta-gal assays working properly!

User Reviews

UNIQ194f3d89b26e2de2-partinfo-00000000-QINU UNIQ194f3d89b26e2de2-partinfo-00000001-QINU Both T9002 and K498000 HSL-detector devices were ligated into one of three different plasmids during the course of our work. T9002 it its original plasmid was used to construct K498000 by incorporating the I172017 (Lac Z) part. Both original T9002 and the composite part K498000 were used to successfully transform Agrobacteria by electroporation (Micro Pulser Electroporator, Bio-Rad protocol). The composite part K498000 was also reconstructed in both pSB1AT3 and pSB1C1 independently through the A3 assembly method. Agrobacteria was successfully transformed with all of the constructs. Since chloramphenicol and tetracycline are protein synthesis inhibitors, all HSL-stimulation experiments were done in pSB1AT3 with ampicillin selection.



IvyTech-South_Bend_Experience.png