Difference between revisions of "Part:BBa K310002"
 
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__NOTOC__
 
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<partinfo>BBa_K310002 short</partinfo>
 
<partinfo>BBa_K310002 short</partinfo>
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[[Image:MicF_Biobrick_mFOLD_small.png|thumb|mFOLD for this sRNA]][[Image:3_sRNA.png|thumb|Targeted Regulation by sRNAs:micF, micA, and gadY.  sRNAs expressed under lac promoter.]]
  
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micF is a small non-coding RNA regulator that binds to the 5' UTR of ompF mRNA transcript via non-perfect base pairing. The primary action of micF is the translational repression of ompF by ribosome occlusion of ompF mRNA and subsequent destabalization of the transcript. micF binds RNA chaperone, hfq, which works to stabalize micA in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex, and specifies cellular localization of sRNA-mRNA interactions.
 
  
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[[Image:MicF_Biobrick_mFOLD_small.png|thumb|mFOLD for this sRNA]]
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micF is a small non-coding RNA regulator that binds to the 5' UTR of ompF mRNA transcript via non-perfect base pairing. The primary action of micF is the translational repression of ompF by ribosome occlusion of ompF mRNA and subsequent destabalization of the transcript. micF binds RNA chaperone, hfq, which works to stabalize micF in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex, and specifies cellular localization of sRNA-mRNA interactions.
  
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===Usage and Biology===
 
===Usage and Biology===
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micF sRNA is a post-transcriptional regulator.  Using in conjunction with transcriptional regulators yield clear and strong bistability of target gene expression (data not shown).  Endogenous to E. coli, micF regulates the expression of the outer membrane proteins in response to environmental stress conditions.
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'''note'''
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-8 nucleotides have been added to the 5' of this sRNA as an artifact of the RFC10 Biobrick assembly (scar coding).
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-overexpression of sRNA to target mRNA yields optimal results (10 sRNA:1 mRNA)
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-overexpression of hfq may affect efficacy of sRNA regulation, but pleiotropic effects observed in cell growth.
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-actual biobrick is much longer than the length of the sRNA; the suffix was placed downsteam of the sRNA intrinsic terminator.
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Latest revision as of 08:12, 6 November 2010

MicF (sRNA)

mFOLD for this sRNA
Targeted Regulation by sRNAs:micF, micA, and gadY. sRNAs expressed under lac promoter.


micF is a small non-coding RNA regulator that binds to the 5' UTR of ompF mRNA transcript via non-perfect base pairing. The primary action of micF is the translational repression of ompF by ribosome occlusion of ompF mRNA and subsequent destabalization of the transcript. micF binds RNA chaperone, hfq, which works to stabalize micF in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex, and specifies cellular localization of sRNA-mRNA interactions.

Usage and Biology

micF sRNA is a post-transcriptional regulator. Using in conjunction with transcriptional regulators yield clear and strong bistability of target gene expression (data not shown). Endogenous to E. coli, micF regulates the expression of the outer membrane proteins in response to environmental stress conditions.

note

-8 nucleotides have been added to the 5' of this sRNA as an artifact of the RFC10 Biobrick assembly (scar coding).

-overexpression of sRNA to target mRNA yields optimal results (10 sRNA:1 mRNA)

-overexpression of hfq may affect efficacy of sRNA regulation, but pleiotropic effects observed in cell growth.

-actual biobrick is much longer than the length of the sRNA; the suffix was placed downsteam of the sRNA intrinsic terminator.











Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]