Difference between revisions of "Part:BBa K313000"

Latest revision as of 09:52, 3 November 2010

gfp generator with T7 promoter

gfp generator under the control of T7 promoter.

T7 RNA polymerase induces the expression of gfp from this part. This part can be used as reporter to monitor the expression of T7 polymerase.

(Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page for detail.)

Plasmid containing this part was transformed into Rosetta (DE3) plysS strain, which have T7 polymerase under the control of Lac promoter.

When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM. Fluorescence was measured and the results are shown below:

Although we observed fluorescence without IPTG induction, the fluorescence intensity was half compared to that of IPTG induced sample.

As a result we successfully confirmed the proper operation of this part.

Sequence and Features

Assembly Compatibility:

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K313000 short</partinfo>
-gfp generator with T7 promoter
+gfp generator under the control of T7 promoter.
 ===Usage and Biology===
-T7 RNA polymerase induces the expression of gfp from this part.
+T7 RNA polymerase induces the expression of gfp from this part. This part can be used as reporter to monitor the expression of T7 polymerase.
-Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.
 === Verification Experiment ===
+(Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page for detail.)
-Plasmid containing this part was transformed to Rosetta (DE3) plysS.
+Plasmid containing this part was transformed into Rosetta (DE3) plysS strain, which have T7 polymerase under the control of Lac promoter.
-When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM
+When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM.
 Fluorescence was measured and the results are shown below:
 [[Image:Graph_5.png]]
+Although we observed fluorescence without IPTG induction, the fluorescence intensity was half compared to that of IPTG induced sample.
+As a result we successfully confirmed the proper operation of this part.
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