Difference between revisions of "Part:BBa K371019:Design"
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===Design Notes=== | ===Design Notes=== | ||
To get this BioBrick, we split this part into five part: promotor, pduAB, pduJK, pduN, pduTUV.Promotor BBa_R0010 comes from parts.igem and was chosen to give a mediate transcription efficiency after induced by IPTG. Pdu AB, pduJK, pduN and pduTUV come from ''Citrobacter Freundii'' genome by Polymerase Chain Reaction(PCR) and the inappropri ate restriction sites were moved before continuing next step assembling. Here is the assembling map of our project. | To get this BioBrick, we split this part into five part: promotor, pduAB, pduJK, pduN, pduTUV.Promotor BBa_R0010 comes from parts.igem and was chosen to give a mediate transcription efficiency after induced by IPTG. Pdu AB, pduJK, pduN and pduTUV come from ''Citrobacter Freundii'' genome by Polymerase Chain Reaction(PCR) and the inappropri ate restriction sites were moved before continuing next step assembling. Here is the assembling map of our project. | ||
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| + | [[Image:PduABJKNTUV_RFC_10_assembly.png|900px]] | ||
===Source=== | ===Source=== | ||
Latest revision as of 12:57, 31 October 2010
pdu(Propanediol utilization)BMC shell gene under Plac control
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1894
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3236
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To get this BioBrick, we split this part into five part: promotor, pduAB, pduJK, pduN, pduTUV.Promotor BBa_R0010 comes from parts.igem and was chosen to give a mediate transcription efficiency after induced by IPTG. Pdu AB, pduJK, pduN and pduTUV come from Citrobacter Freundii genome by Polymerase Chain Reaction(PCR) and the inappropri ate restriction sites were moved before continuing next step assembling. Here is the assembling map of our project.
Source
Citrobactor freudii, the strain was bought from NITE Biological Resource Center (NBRC). NBRC NO. is 12681. History:IFO 12681 <- Ajinomoto Co., Inc. (AJ 2619) <- ATCC 8090 <- C.H. Werkm