Difference between revisions of "Part:BBa K339003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Obtained in plasmid form from the Betton labs in France. Used PCR to amplify the gene out of the plasmid and add the biobrick prefix and suffix. | + | Obtained in plasmid form from the Betton labs in France (Betton et al., 2002). Used PCR to amplify the gene out of the plasmid and add the biobrick prefix and suffix. |
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===Source=== | ===Source=== | ||
| + | etton, J., Phichith, D. and Hunke, S. (2002). Folding and aggregation of export-defective mutants of the maltose-binding protein. ''Research in Microbiology''. (153): 399-404. | ||
===References=== | ===References=== | ||
Latest revision as of 00:32, 31 October 2010
malE31ΔSS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 360
Illegal BamHI site found at 96 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 58
Design Notes
Obtained in plasmid form from the Betton labs in France (Betton et al., 2002). Used PCR to amplify the gene out of the plasmid and add the biobrick prefix and suffix.
Source
etton, J., Phichith, D. and Hunke, S. (2002). Folding and aggregation of export-defective mutants of the maltose-binding protein. Research in Microbiology. (153): 399-404.