Difference between revisions of "Part:BBa K415505"

Revision as of 02:41, 29 October 2010

VgEcR_2A_RxR L1L2 MammoBlock Entry Vector

Figure 1. Schematic of PonS Induction of the EGSH promoter, leading to transcriptional activation.

Schematic: Response of EGSH to the addition of PonS, in the presence of VgEcR and RxR.

Mammalian L1L2 Entry Vector containing VgEcR_2A_RxR construct. retinoid X receptor (RxR) and ecdysone receptors (VgEcR) form a heterodimer to control expression of the EGSH promoter; they can be activated by the addition of the PonS compound. Here they are separated by a viral 2A site that allows for bicistronic expression. (Figure 1)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 291
Illegal PstI site found at 2272
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2091
Illegal PstI site found at 2272
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1585
Illegal BamHI site found at 486
Illegal BamHI site found at 2962
Illegal BamHI site found at 3115
Illegal BamHI site found at 3229
Illegal XhoI site found at 1578
Illegal XhoI site found at 1659
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 291
Illegal PstI site found at 2272
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 291
Illegal PstI site found at 2272
Illegal NgoMIV site found at 1707
Illegal NgoMIV site found at 2054
Illegal NgoMIV site found at 3527
Illegal AgeI site found at 2815
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 3000
Illegal SapI.rc site found at 389
Illegal SapI.rc site found at 521

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K415505 short</partinfo>
-Mammalian L1L2 Entry Vector containing VgEcR_2A_RxR construct. VgEcR and RxR form a heterodimer to control expression of the EGSH promoter; they can be activated by the addition of the PonS compound. Here they are separated by a viral 2A site that allows for bicistronic expression.
+[[Image:EGSH-logic.jpg|thumb|left|Figure 1. Schematic of PonS Induction of the EGSH promoter, leading to transcriptional activation.]] '''Schematic: Response of EGSH to the addition of PonS, in the presence of VgEcR and RxR.'''
+Mammalian L1L2 Entry Vector containing VgEcR_2A_RxR construct. retinoid X receptor (RxR) and ecdysone receptors (VgEcR) form a heterodimer to control expression of the EGSH promoter; they can be activated by the addition of the PonS compound. Here they are separated by a viral 2A site that allows for bicistronic expression. (Figure 1)
+&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 <!-- Add more about the biology of this part here