Difference between revisions of "Part:BBa K415507"

Revision as of 02:25, 29 October 2010

pEGSH L4R1 MammoBlock Entry Vector

Figure 1. Schematic of PonS Induction of the EGSH promoter, leading to transcriptional activation.

Schematic: Response of K415507 to the addition of PonS, in the presence of VgEcR and RxR.

L4R1 MammoBlock entry vector. EGSH is an inducible promoter. In the presence of the proteins retinoid X receptor (RxR) and ecdysone receptors (VgEcR), the addition of ponasterone S (PonS) leads to complex formation of RxR, VgEcR, and PonS. This activated complex is then capable of inducing the EGSH promoter and allows for dramatic upregulation in transcriptional activity.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal BglII site found at 115
Illegal BamHI site found at 663
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 646

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K415507 short</partinfo>
-L4R1 MammoBlock entry vector containing the EGSH ecdysone-inducible promoter. This promoter is controlled by a heterodimer between the VgEcR and RxR proteins.
+[[Image:EGSH-logic.jpg|thumb|left|Figure 1. Schematic of PonS Induction of the EGSH promoter, leading to transcriptional activation.]] '''Schematic: Response of K415507 to the addition of PonS, in the presence of VgEcR and RxR.'''
-<!-- Add more about the biology of this part here
+L4R1 MammoBlock entry vector. EGSH is an inducible promoter. In the presence of the proteins retinoid X receptor (RxR) and ecdysone receptors (VgEcR), the addition of ponasterone S (PonS) leads to complex formation of RxR, VgEcR, and PonS. This activated complex is then capable of inducing the EGSH promoter and allows for dramatic upregulation in transcriptional activity.
-===Usage and Biology===
-<!-- -->
+&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-<span class='h3bb'>Sequence and Features</span>
+==Sequence and Features==
 <partinfo>BBa_K415507 SequenceAndFeatures</partinfo>