Difference between revisions of "Part:BBa K371019"

Revision as of 01:10, 29 October 2010

pdu(Propanediol utilization)BMC shell gene under Plac control

This composite consists of eight pdu bacterial microcompartment(BMC) structure gene following a natural lac promotor(BBa_R0010). These genes come from four basic parts,pduAB,pduJK,pduN and pduTUV, which consist of the natural RBS and gene separately.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 3236
1000
COMPATIBLE WITH RFC[1000]

In the expression of protein, we use and unite the method mentioned in the previous papers. [see protocol] The bacterial was induced by 1 mM IPTG at 30 ℃ for 4 hours. The results was as follows.[the one without pduN was a negative control used to prove the function of pduN: in the growth process, the one without pduN tends to grow with more deposit implying its possible role in the vertex] We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet. [[1]]

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 <partinfo>BBa_K371019 parameters</partinfo>
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+In the expression of protein, we use and unite the method mentioned in the previous papers. [see protocol] The bacterial was induced by 1 mM IPTG at 30 ℃ for 4 hours. The results was as follows.[the one without pduN was a negative control used to prove the function of pduN: in the growth process, the one without pduN tends to grow with more deposit implying its possible role in the vertex]
 [[Image:Before_iptg..jpg]]
+We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet.
+[[Image:Pdu_Sds-page.jpg]]
 [[https://static.igem.org/mediawiki/2010/c/c3/Pdu_Sds-page.jpg]]