Difference between revisions of "Part:BBa K352017"

 
 
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<partinfo>BBa_K352017 short</partinfo>
 
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This part is formed by combination of pCooM Promoter coupled with RBS+RFP+Double terminator and CooA coupled with placI+RBS.
 
This part is formed by combination of pCooM Promoter coupled with RBS+RFP+Double terminator and CooA coupled with placI+RBS.
  
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<br> This component is used to measure high levels of carbon monoxide in the environment. Construction of this device was performed by utilizing strong CO sensitive promoter which is pCooM promoter from Rhodospirillum rubrum. This device is only active when there is CooA transcriptional activator and Carbon monoxide gas in the environment. When there is CO in the environment, the device produces red fluorescent protein in response.
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===Usage and Biology===
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<br>  <html><div><a href="https://parts.igem.org/Part:BBa_K352017:Experience"><strong>Click for Details...</strong></a></div></html>
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==Culturing CO Sensor E.coli with CO induction==
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<h3>Usage and Biology</h3>
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<html><div  align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 500px; height: 200px;" alt="w7" src="https://static.igem.org/mediawiki/2010/4/49/Culturing_alpha_1.JPG"></a></div> </html>
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<br> We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 00:09, 29 October 2010

pCooM RBS RFP Double terminator CooA coupled with placI+RBS

This part is formed by combination of pCooM Promoter coupled with RBS+RFP+Double terminator and CooA coupled with placI+RBS.


This component is used to measure high levels of carbon monoxide in the environment. Construction of this device was performed by utilizing strong CO sensitive promoter which is pCooM promoter from Rhodospirillum rubrum. This device is only active when there is CooA transcriptional activator and Carbon monoxide gas in the environment. When there is CO in the environment, the device produces red fluorescent protein in response.




Culturing CO Sensor E.coli with CO induction

Usage and Biology

w7


We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
    Illegal AgeI site found at 759
    Illegal AgeI site found at 1617
  • 1000
    COMPATIBLE WITH RFC[1000]