Difference between revisions of "Part:BBa K415511"

 
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__NOTOC__
 
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<partinfo>BBa_K415511 short</partinfo>
 
<partinfo>BBa_K415511 short</partinfo>
  
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MIT iGEM 2010 Mammalian Part
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L4R1 MammoBlock Promoter Vector. Contains plasminogen activator inhibitor 2 (PAI-2) promoter, shown to increase transcriptional activity in mammalian cells in response to radial and circumferential cellular strain on a cell monolayer
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:59, 28 October 2010

pPAI2 L4R1 MammoBlock Entry Vector

L4R1 MammoBlock Promoter Vector. Contains plasminogen activator inhibitor 2 (PAI-2) promoter, shown to increase transcriptional activity in mammalian cells in response to radial and circumferential cellular strain on a cell monolayer

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal XbaI site found at 145
    Illegal SpeI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal SpeI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal BglII site found at 1082
    Illegal BglII site found at 1402
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal XbaI site found at 145
    Illegal SpeI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal XbaI site found at 145
    Illegal SpeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1313
    Illegal BsaI site found at 1578
    Illegal BsaI.rc site found at 674
    Illegal SapI.rc site found at 1016