Difference between revisions of "Part:BBa K398326:Experience"
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| + | In order to convert from AU of fluorescence to GFP molecules we used the conversion factor reported for the part [https://parts.igem.org/Part:BBa_E0040 BBa_E0040] which is: 79.429 nM^-1 according to the page of E0040. Nevertheless, we believe that this conversion factor it is also dependent on the path length... We couldn't find information about the path length that the people that characterize E0040 used, thus we are just using the conversion factor ASSUMING THAT THERE'S NO PATH LENGTH DEPENDENCY. In case that you prove that indeed there is a path length dependecy, we use a microtiter plate of 300 uL with a total reaction volume of 100 uL. Then, we ask you to correct the values found. | ||
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Revision as of 14:09, 28 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K398326
- iGEM TU Delft 2010
In order to measure the strength (efficiency) of this promoter we built the part K938331 which is pCaiF+ E0240. Check [http://2010.igem.org/Team:TU_Delft#page=Project/sensing/results our results page] in order to know more about our experience with this part!!!
| Condition | Exponential phase [GFP molecules/O.D.] | Stationary phase [GFP molecules/O.D.] |
|---|---|---|
| LB | 1.2516E+07 | -8.6245E+05 |
| 0.5LB | 1.8101E+07 | -2.5945E+05 |
| [glc]=10g/L | 6.6456E+06 | -6.7109E+06 |
| [glc]=5g/L | 8.3673E+06 | 4.2339E+06 |
| [glc]=2g/L | 7.2869E+06 | 3.9755E+07 |
| Diauxic growth (glucose phase) | 7.4517E+06 | 2.1543E+07 |
| Diauxic growth (Laurate phase) | 1.1435E+07 | 6.6428E+06 |
Also a simple model for this system was developed. [http://2010.igem.org/Team:TU_Delft#page=Modeling/pcaif-model Click here] in order to know more about it!.
User Reviews
In order to convert from AU of fluorescence to GFP molecules we used the conversion factor reported for the part BBa_E0040 which is: 79.429 nM^-1 according to the page of E0040. Nevertheless, we believe that this conversion factor it is also dependent on the path length... We couldn't find information about the path length that the people that characterize E0040 used, thus we are just using the conversion factor ASSUMING THAT THERE'S NO PATH LENGTH DEPENDENCY. In case that you prove that indeed there is a path length dependecy, we use a microtiter plate of 300 uL with a total reaction volume of 100 uL. Then, we ask you to correct the values found.
UNIQb4398a5a554471d1-partinfo-00000000-QINU UNIQb4398a5a554471d1-partinfo-00000001-QINU