Difference between revisions of "Part:BBa K313035"
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This is a device that aims to test the recombination ability of the Hin DNA invertase. | This is a device that aims to test the recombination ability of the Hin DNA invertase. | ||
| − | Two hixCs are arranged so that the intermediate double terminator is cut out and the RFP begins to express when Hin is expressed. So, this unit can be used as a detector of Hin, because cells with this construct will | + | Two hixCs are arranged so that the intermediate double terminator is cut out and the RFP begins to express when Hin is expressed. So, this unit can be used as a detector of Hin, because cells with this construct will look red only when the cells express Hin. |
| + | Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.<br/> | ||
Latest revision as of 03:55, 28 October 2010
Hin invertase assay device
This is a device that aims to test the recombination ability of the Hin DNA invertase.
Two hixCs are arranged so that the intermediate double terminator is cut out and the RFP begins to express when Hin is expressed. So, this unit can be used as a detector of Hin, because cells with this construct will look red only when the cells express Hin.
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 798
Illegal AgeI site found at 910 - 1000COMPATIBLE WITH RFC[1000]