Difference between revisions of "Part:BBa K313036"

 
 
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<partinfo>BBa_K313036 short</partinfo>
 
<partinfo>BBa_K313036 short</partinfo>
  
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Loading sequence deficient MS2 phage cDNA.  
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This part is a loading sequence-and-replicase-deficient version of the cDNA of phage MS2, BBa_K313006. (XbaI site is removed.)
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Virus transformed from this part carry out mRNA with specific loading sequence(19bp)
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and infect another E.coli.
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This cDNA does not have the loading sequence, BBa_K313010, which is necessary for loading of RNA into the capsid of MS2 phage. So, MS2 phage produced from this part cannot load the RNA of itself. Instead, the capsid is assumed to load any mRNAs which includes the loading sequence.
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In the infected E.coli, translation of mRNA occur.  
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This mutant phage will be able to carry mRNAs you want to various cells as information.
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As this mutant phage has lost the coding region of its replicase, its arbitrary proliferation will be prevented.
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Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2  Phage MS2] assay page.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:52, 28 October 2010

MS2 phage cDNA (loading sequence and replicase-deficient)

This part is a loading sequence-and-replicase-deficient version of the cDNA of phage MS2, BBa_K313006. (XbaI site is removed.)

This cDNA does not have the loading sequence, BBa_K313010, which is necessary for loading of RNA into the capsid of MS2 phage. So, MS2 phage produced from this part cannot load the RNA of itself. Instead, the capsid is assumed to load any mRNAs which includes the loading sequence. This mutant phage will be able to carry mRNAs you want to various cells as information.

As this mutant phage has lost the coding region of its replicase, its arbitrary proliferation will be prevented.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2 Phage MS2] assay page.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
    Illegal BamHI site found at 2057
    Illegal XhoI site found at 1048
    Illegal XhoI site found at 3214
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 1733
    Illegal NgoMIV site found at 2437
    Illegal NgoMIV site found at 3309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1665
    Illegal BsaI.rc site found at 163
    Illegal BsaI.rc site found at 2257