Difference between revisions of "Part:BBa K313021"
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<partinfo>BBa_K313021 short</partinfo> | <partinfo>BBa_K313021 short</partinfo> | ||
| − | This is a device that aims to measure the leakiness of | + | This is a device that aims to measure the leakiness of a terminator BBa_B0017. |
If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. | If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. | ||
With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator. | With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator. | ||
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| + | Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page. | ||
Latest revision as of 03:40, 28 October 2010
terminator leakiness assay device
This is a device that aims to measure the leakiness of a terminator BBa_B0017.
If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 479
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 165
- 1000COMPATIBLE WITH RFC[1000]