Difference between revisions of "Part:BBa K319005:Design"
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===Source=== | ===Source=== | ||
− | The part was extracted from S. cerevisiae | + | The part was extracted from ''S. cerevisiae'' YPH500 strain using colony PCR protocols. It consists of 720 bp upstream of ADH1 gene. The primers were designed according to the genomic sequence found on [http://www.yeastgenome.org ''Saccharomyces'' Genome Database]. The design, extraction, and cloning the BioBrick part were performed by Afnan Azizi of 2010 uOttawa iGEM team. |
===References=== | ===References=== |
Latest revision as of 03:33, 28 October 2010
yeast mid-length ADH1 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 93
Design Notes
There were three options for the length of the promoter - short, middle, and long. The middle length was chosen.
Source
The part was extracted from S. cerevisiae YPH500 strain using colony PCR protocols. It consists of 720 bp upstream of ADH1 gene. The primers were designed according to the genomic sequence found on [http://www.yeastgenome.org Saccharomyces Genome Database]. The design, extraction, and cloning the BioBrick part were performed by Afnan Azizi of 2010 uOttawa iGEM team.