Difference between revisions of "Part:BBa K341456:Design"
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===Source=== | ===Source=== | ||
| − | Kanamycin resistance gene is form plasmid "pKD13" | + | Kanamycin resistance gene is form plasmid "pKD13" which is generously provided by Guoqiang CHEN of Microorganism Lab in Tsinghua University |
===References=== | ===References=== | ||
Revision as of 02:51, 28 October 2010
Kanamycin resistance(with Promoter) + I-sceI site+ Recombination site
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 893
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 229
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 169
Illegal SapI site found at 379
Illegal SapI site found at 1225
Design Notes
This part involves DraIII cutting site at two ends of the unit, this cutting site is compatible with BBF RFC 61
Thsi part is uesd as a Insertion Fragment of Donor Plasmid in the In-vivo Recombination System of iGEM Tsinghua 2010 Project.
Donor Plasmid contains several Insertion Fragments and each Insertion Fragment contains following five parts:
1) one 15bp-long restriction enzyme I-scel recognition site
2) one 25bp-long random sequence
3) one fragment for insertion and recombination
4) one 25bp-long random sequence
5) one 15bp-long restriction enzyme I-scel recognition site(in correspondence with 1)
Donor plasmid contains several Insertion Fragments. Based on the flanking landing pad sequence, we can ascribe Insertion Fragments to the same group as long as the flanking landing pad sequences of those Insertion Fragments are the same.
In order to achieve different goals, we design different Donor Plasmids, different Donor Plasmids contain different number of Insertion Fragments, which belong to different groups.
Source
Kanamycin resistance gene is form plasmid "pKD13" which is generously provided by Guoqiang CHEN of Microorganism Lab in Tsinghua University