Difference between revisions of "Part:BBa K404208"

Latest revision as of 02:44, 28 October 2010

ViralBrick-587-Beta Lactamase (BLA)

ViralBrick-587-Bla
BioBrick Nr.	BBa_K404208
RFC standard	RFC 25
Requirement	pSB1C3
Source
Submitted by	[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]

Usage and Biology

ß-Lactamase (BLA) is an enzyme produced by some bacteria that cleaves the ß-lactam ring of various antibiotics such as penicillin, rendering them inactive. Because the peptides' N- and C-termini are in close proximity to each other, we created a ViralBrick containing BLA to see if capsid assembly still takes place with such a large insert in place. Assembled capsids can easily be detected by a beta-lactamase assay.

Restriction sites

Restriction site	Enzyme	Recognition sequence	Activity	Temp.
upstream 587	[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]		100;50;10;100	37°C
downstream 587	[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]		0;25;0;100	37°C

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 10
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K404208 short</partinfo>
+{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
+! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404208 ViralBrick-587-Bla]
+|-
+|'''BioBrick Nr.'''
+|[https://parts.igem.org/Part:BBa_K404208 BBa_K404208]
+|-
+|'''RFC standard'''
+|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
+|-
+|'''Requirement'''
+|pSB1C3<br>
+|-
+|'''Source'''
+|
+|-
+|'''Submitted by'''
+|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
+|}
+<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 [[Image:Freiburg10_ViralBrick-logo-587-BLA.png|thumb|center|480px]]<br>
 <!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+<html>
+<div style="width:965px; height:400px; ">
+<div style="float:left; width:460px; height:auto;  margin: 0px 5px 0px 5px; text-align:justify;">
+ß-Lactamase (BLA) is an enzyme produced by some bacteria that cleaves the ß-lactam ring of various antibiotics such as penicillin, rendering them inactive. Because the peptides' N- and C-termini are in close proximity to each other, we created a ViralBrick containing BLA to see if capsid assembly still takes place with such a large insert in place.
+Assembled capsids can easily be detected by a beta-lactamase assay.
+</div>
+</html>
+<br><br><br><br><br><br>
 ===Restriction sites===
@@ Line 16: / Line 46: @@
    <th><b>Activity</b></th>
    <th><b>Temp.</b></th>
- </tr>
- <tr>
-  <td>upstream 453</td>
-  <td>[http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF]</td>
-  <td>[[image:Freiburg10_recognition site SspI-HF.Gif]]</td>
-  <td>25;100;0;100</td>
- <td>37°C</td>
   </tr>
 <tr>
-   <td>downstream 453</td>
+   <td>upstream 587</td>
-   <td>[http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF]</td>
+   <td>[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]</td>
-   <td>[[image:Freiburg10_recognition site SalI-HF.Gif]]</td>
+   <td>[[image:Freiburg10_recognition site BamHI-HF.Gif]]</td>
-   <td>10;100;100;100</td>
+   <td>100;50;10;100</td>
    <td>37°C</td>
+ </tr>
+<tr>
+  <td>downstream 587</td>
+  <td>[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]</td>
+  <td>[[image:Freiburg10_recognition site PvuII-HF.Gif]]</td>
+  <td>0;25;0;100</td>
+  <td>37°C
+ </tr>
 <tr>
 </table>
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>