Difference between revisions of "Part:BBa K404213"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K404213 short</partinfo> | <partinfo>BBa_K404213 short</partinfo> | ||
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− | + | {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left" | |
+ | ! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404165 pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty)] | ||
+ | |- | ||
+ | |'''BioBrick Nr.''' | ||
+ | |[https://parts.igem.org/Part:BBa_K404165 BBa_K404165] | ||
+ | |- | ||
+ | |'''RFC standard''' | ||
+ | |[https://parts.igem.org/Help:Assembly_standard_10 RFC 10] | ||
+ | |- | ||
+ | |'''Requirement''' | ||
+ | |pSB1C3<br> | ||
+ | |- | ||
+ | |'''Source''' | ||
+ | | | ||
+ | |- | ||
+ | |'''Submitted by''' | ||
+ | |[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] | ||
+ | |} | ||
+ | <br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/> | ||
+ | <br><b>The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop</b> | ||
+ | |||
+ | [[Image:Freiburg10_ViralBrick-logo-587KO-Z34C.png|thumb|center|480px]]<br> | ||
+ | |||
+ | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <html> | ||
+ | <div style="width:965px; height:400px; "> | ||
+ | <div style="float:left; width:460px; height:auto; margin: 0px 5px 0px 5px; text-align:justify;"> | ||
+ | The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of <a href=http://www.ncbi.nlm.nih.gov/protein/153106>Staphylococcal Protein A</a>. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering. | ||
+ | |||
+ | </div> | ||
+ | <div style="float:right; width:480px; height:auto; "><img src="https://static.igem.org/mediawiki/parts/9/98/Freiburg10_ViralBrick_motif_Z34C.png" width="460" | ||
+ | height="auto"/></div></div> | ||
+ | </html> | ||
+ | |||
<!-- --> | <!-- --> | ||
+ | |||
+ | ===Restriction sites=== | ||
+ | <table border="1" style="float:right; width:480px; height:auto;> | ||
+ | <tr> | ||
+ | <th><b>Restriction site</b></th> | ||
+ | <th><b>Enzyme</b></th> | ||
+ | <th><b>Recognition sequence</b></th> | ||
+ | <th><b>Activity</b></th> | ||
+ | <th><b>Temp.</b></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>upstream 587</td> | ||
+ | <td>[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]</td> | ||
+ | <td>[[image:Freiburg10_recognition site BamHI-HF.Gif]]</td> | ||
+ | <td>100;50;10;100</td> | ||
+ | <td>37°C</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>downstream 587</td> | ||
+ | <td>[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]</td> | ||
+ | <td>[[image:Freiburg10_recognition site PvuII-HF.Gif]]</td> | ||
+ | <td>0;25;0;100</td> | ||
+ | <td>37°C | ||
+ | </tr> | ||
+ | <tr> | ||
+ | </table> | ||
+ | |||
+ | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K404213 SequenceAndFeatures</partinfo> | <partinfo>BBa_K404213 SequenceAndFeatures</partinfo> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Latest revision as of 02:43, 28 October 2010
ViralBrick-587KO-Z34C
pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty) | |
---|---|
BioBrick Nr. | BBa_K404165 |
RFC standard | RFC 10 |
Requirement | pSB1C3 |
Source | |
Submitted by | [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] |
The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop
Usage and Biology
The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
Restriction sites
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 10
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]