Difference between revisions of "Part:BBa K404213"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404213 short</partinfo>
 
<partinfo>BBa_K404213 short</partinfo>
 +
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
 +
! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404213 ViralBrick-587KO-Z34C]
 +
|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_K404213 BBa_K404213]
 +
|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
 +
|-
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|'''Requirement'''
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|pSB1C3<br>
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|-
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|'''Source'''
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|
 +
|-
 +
|'''Submitted by'''
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|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
 +
|}
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 +
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
 +
! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404165 pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty)]
 +
|-
 +
|'''BioBrick Nr.'''
 +
|[https://parts.igem.org/Part:BBa_K404165 BBa_K404165]
 +
|-
 +
|'''RFC standard'''
 +
|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
 +
|-
 +
|'''Requirement'''
 +
|pSB1C3<br>
 +
|-
 +
|'''Source'''
 +
|
 +
|-
 +
|'''Submitted by'''
 +
|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
 +
|}
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 
<br><b>The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop</b>
 
<br><b>The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop</b>
  

Revision as of 02:37, 28 October 2010

ViralBrick-587KO-Z34C

ViralBrick-587KO-Z34C
BioBrick Nr. BBa_K404213
RFC standard RFC 25
Requirement pSB1C3
Source
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]














pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty)
BioBrick Nr. BBa_K404165
RFC standard RFC 10
Requirement pSB1C3
Source
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]















The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop

Freiburg10 ViralBrick-logo-587KO-Z34C.png


Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.


Restriction sites

Restriction site Enzyme Recognition sequence Activity Temp.
upstream 587 [http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF] Freiburg10 recognition site BamHI-HF.Gif 100;50;10;100 37°C
downstream 587 [http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF] Freiburg10 recognition site PvuII-HF.Gif 0;25;0;100 37°C


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]