Difference between revisions of "Part:BBa K404204"
 
(4 intermediate revisions by one other user not shown)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404204 short</partinfo>
 
<partinfo>BBa_K404204 short</partinfo>
 +
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
 +
! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404204 ViralBrick-453-Z34C)]
 +
|-
 +
|'''BioBrick Nr.'''
 +
|[https://parts.igem.org/Part:BBa_K404204 BBa_K404204]
 +
|-
 +
|'''RFC standard'''
 +
|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
 +
|-
 +
|'''Requirement'''
 +
|pSB1C3<br>
 +
|-
 +
|'''Source'''
 +
|
 +
|-
 +
|'''Submitted by'''
 +
|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
 +
|}
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 
<br><b>The Z34C antibody binding motif, ready for insertion into the 453 loop</b>
 
<br><b>The Z34C antibody binding motif, ready for insertion into the 453 loop</b>
  
Line 8: Line 27:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the <a href=http://www.ncbi.nlm.nih.gov/protein/153106>Staphylococcal Protein A</a>.  
+
The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of  [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
  
 
[[Image:Freiburg10_ViralBrick_motif_Z34C.png|thumb|center|480px]]
 
[[Image:Freiburg10_ViralBrick_motif_Z34C.png|thumb|center|480px]]
 +
 +
 +
===Restriction sites===
 +
 +
<table border="1" style="float:right; width:480px; height:auto;>
 +
<tr>
 +
  <th><b>Restriction site</b></th>
 +
  <th><b>Enzyme</b></th>
 +
  <th><b>Recognition sequence</b></th>
 +
  <th><b>Activity</b></th>
 +
  <th><b>Temp.</b></th>
 +
</tr>
 +
<tr>
 +
  <td>upstream 453</td>
 +
  <td>[http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF]</td>
 +
  <td>[[image:Freiburg10_recognition site SspI-HF.Gif]]</td>
 +
  <td>25;100;0;100</td>
 +
<td>37°C</td>
 +
</tr>
 +
<tr>
 +
  <td>downstream 453</td>
 +
  <td>[http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF]</td>
 +
  <td>[[image:Freiburg10_recognition site SalI-HF.Gif]]</td>
 +
  <td>10;100;100;100</td>
 +
  <td>37°C</td>
 +
 +
<tr>
 +
</table>
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 02:21, 28 October 2010

ViralBrick-453-Z34C

ViralBrick-453-Z34C)
BioBrick Nr. BBa_K404204
RFC standard RFC 25
Requirement pSB1C3
Source
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]















The Z34C antibody binding motif, ready for insertion into the 453 loop

Freiburg10 ViralBrick-logo-453-Z34C.png


Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Freiburg10 ViralBrick motif Z34C.png


Restriction sites

Restriction site Enzyme Recognition sequence Activity Temp.
upstream 453 [http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF] Freiburg10 recognition site SspI-HF.Gif 25;100;0;100 37°C
downstream 453 [http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF] Freiburg10 recognition site SalI-HF.Gif 10;100;100;100 37°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]