Difference between revisions of "Part:BBa K404319"
 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
 
<br/><br><br>
 
<br/><br><br>
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
−
! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404319 [Cyan fluorescent protein]
+
! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404319 Cyan fluorescent protein]
 
|-
 
|-
 
|'''BioBrick Nr.'''
 
|'''BioBrick Nr.'''
Line 26: Line 26:
 
<html>
 
<html>
 
Nearly all cyan fluorescent proteins are generated on the basis of GFP-like proteins from jellyfish Aequorea macrodactyla.  
 
Nearly all cyan fluorescent proteins are generated on the basis of GFP-like proteins from jellyfish Aequorea macrodactyla.  
−
mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has a higher brightness level, acid insensivity and greater photostability.  
+
mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has higher brightness level, acid insensivity and greater photostability.  
 
Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles.   
 
Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles.   
 
<html/><br/>
 
<html/><br/>

Latest revision as of 01:26, 28 October 2010

Cyan fluorescent protein (CFP) in RFC25 standard


Cyan fluorescent protein
BioBrick Nr. BBa_K404319
RFC standard RFC 25
Requirement pSB1C3
Source
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]













Nearly all cyan fluorescent proteins are generated on the basis of GFP-like proteins from jellyfish Aequorea macrodactyla. mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has higher brightness level, acid insensivity and greater photostability. Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles.


 

 

 

                                        

 

Fig.1: Emission spectra and absorbance CFP

 

http://www.olympusmicro.com/primer/techniques/fluorescence/fret/images/fretintrofigure4.jpg

 

 

 

 

 

                             
 

   

  

 

 

 

 

 

 

 

 

 

 

 

 


Fig.2:Chromophor structure of CFP

http://jcs.biologists.org/cgi/reprint/120/24/4247

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


Fig.3:  Fluorescent protein beta-barrel architecture and approximate dimensions

http://jcs.biologists.org/cgi/reprint/120/24/4247

 

 


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]