Difference between revisions of "Templates/Cambridge-fireflycolour"

Latest revision as of 01:04, 28 October 2010

Input:	L-Arabinose
Output:	Light ({{{colour}}})

pBad/araC I0500	mutated version of Luciferase/LRE K325210

This part should show similarity to BBa_K325219 [[Part:BBa_K325{{{id}}}:Experience |Add Data]]

Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.

Colour of emission

Figure 1 - Comparison of part BBa_K325{{{id}}} against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3

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 {|
-|width="226px" align="center" valign="center"|<span style="font-family: Arial; font-size: 12pt">
+|width="326px" align="center" valign="center"|<span style="font-family: Arial; font-size: 12pt">
 {|
 |width="60px"|''Input'':
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-==Pictures==
+==Colour of emission==
-[[Image:300px-Cambridge-Wed.jpg|thumb|569px|center|'''Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with [https://parts.igem.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [https://parts.igem.org/Part:BBa_K325219 BBa 325219] (red light bulb) ''']]
+[[Image:Rainbow.png|thumb|569px|center|Figure 1 - Comparison of part BBa_K325{{{id}}} against other constructs created by the E.glowli team.  Note that this picture should be used as a relative comparison rather than absolute colour.  All images change markedly depending on the white balance used.]]
-<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]