Difference between revisions of "Templates/Cambridge-fireflycolour"

Latest revision as of 01:04, 28 October 2010

Input:	L-Arabinose
Output:	Light ({{{colour}}})

pBad/araC I0500	mutated version of Luciferase/LRE K325210

This part should show similarity to BBa_K325219 [[Part:BBa_K325{{{id}}}:Experience |Add Data]]

Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.

Colour of emission

Figure 1 - Comparison of part BBa_K325{{{id}}} against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3

@@ Line 1: / Line 1: @@
-<div style="font-family: Arial; padding: 00px; width: 680px; border: 0px solid #000000;">
 {|
-|width="226px" align="center" valign="center"|<span style="font-family: Arial; font-size: 12pt">
+|width="326px" align="center" valign="center"|<span style="font-family: Arial; font-size: 12pt">
 {|
 |width="60px"|''Input'':
@@ Line 7: / Line 6: @@
 |-
 |''Output'':
-| Light {{{colour}}}
+| Light ({{{colour}}})
 |}
 </span>
-|width="226px" align="center" valign="center"|<html><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;width:200px'>pBad/araC<br>I0500</TD><TD style='font-size:90%; line-height:100%;text-align:center;'><strong>mutated version of</strong><br>Luciferase/LRE<br>K325210</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
+|width="326px" align="center" valign="center"|<html><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;width:200px'>pBad/araC<br>I0500</TD><TD style='font-size:90%; line-height:100%;text-align:center;'><strong>mutated version of</strong><br>Luciferase/LRE<br>K325210</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
 promoter (tetR, negative)' src='https://parts.igem.org/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
 luxR Protein Generator' src='https://parts.igem.org/images/partbypart/icon_reporter.png' height='25' border=0></A></TABLE></html>
-|width="226px" align="center" valign="center"|[[Image:BBa_F2620Icon.png|200px]]
+|width="356px" align="right" valign="center"|<html><img src="https://static.igem.org/mediawiki/parts/c/c3/Cambridge-Eglowli.png" /></html>
 |}
-<hr width="680px">
+<hr >
-<div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0; width: 660px; align: center">
+<div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0;  align: center">
 <center>
 <span style="color:black">This part should show similarity to</span> [https://parts.igem.org/Part:BBa_K325219 BBa_K325219]&nbsp;&nbsp;&nbsp;&nbsp; [[Part:BBa_K325{{{id}}}:Experience |Add Data]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
@@ Line 23: / Line 22: @@
 </div><br>
-<div style="padding: 00px; width: 680px; border: 0px solid #000000;">
 '''Description'''<br>
-This part is based on part [https://parts.igem.org/Part:BBa_K325219 BBa_K325219].  Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part [https://parts.igem.org/Part:BBa_K325209 BBa_K325209] which is the green wild-type luciferase].  Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.
+This part is based on part [https://parts.igem.org/Part:BBa_K325219 BBa_K325219].  Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part [https://parts.igem.org/Part:BBa_K325209 BBa_K325209] which is the green wild-type luciferase.  Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.
-==Pictures==
+==Colour of emission==
+[[Image:Rainbow.png|thumb|569px|center|Figure 1 - Comparison of part BBa_K325{{{id}}} against other constructs created by the E.glowli team.  Note that this picture should be used as a relative comparison rather than absolute colour.  All images change markedly depending on the white balance used.]]
-[[Image:300px-Cambridge-Wed.jpg|thumb|569px|center|'''Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with [https://parts.igem.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [https://parts.igem.org/Part:BBa_K325219 BBa 325219] (red light bulb) ''']]
-<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
-<div style="padding: 00px; width: 680px">
-==Sequences==
-<partinfo>BBa_K325{{{id}}} SequenceAndFeatures</partinfo>
 '''Compatibility'''<br>
 [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br>
 [[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
-</div>