Difference between revisions of "Templates/Cambridge-fireflycolour"
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{|
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|width="226px" align="center" valign="center"|<span style="font-family: Arial; font-size: 12pt">
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|width="326px" align="center" valign="center"|<span style="font-family: Arial; font-size: 12pt">
 
{|
 
{|
 
|width="60px"|''Input'':
 
|width="60px"|''Input'':
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|''Output'':
 
|''Output'':
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| Light {{{colour}}}
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| Light ({{{colour}}})
 
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|width="226px" align="center" valign="center"|<html><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;width:200px'>pBad/araC<br>I0500</TD><TD style='font-size:90%; line-height:100%;text-align:center;'><strong>mutated version of</strong><br>Luciferase/LRE<br>K325210</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>lux pR<br>R0062</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
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|width="326px" align="center" valign="center"|<html><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;width:200px'>pBad/araC<br>I0500</TD><TD style='font-size:90%; line-height:100%;text-align:center;'><strong>mutated version of</strong><br>Luciferase/LRE<br>K325210</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
 
promoter (tetR, negative)' src='https://parts.igem.org/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
 
promoter (tetR, negative)' src='https://parts.igem.org/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
 
luxR Protein Generator' src='https://parts.igem.org/images/partbypart/icon_reporter.png' height='25' border=0></A></TABLE></html>
 
luxR Protein Generator' src='https://parts.igem.org/images/partbypart/icon_reporter.png' height='25' border=0></A></TABLE></html>
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|width="226px" align="center" valign="center"|[[Image:BBa_F2620Icon.png|200px]]
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|width="356px" align="right" valign="center"|<html><img src="https://static.igem.org/mediawiki/parts/c/c3/Cambridge-Eglowli.png" /></html>
 
|}
 
|}
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<hr width="680px">
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<hr >
  
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<div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0; width: 660px; align: center">
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<div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0; align: center">
 
<center>
 
<center>
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[[Part:BBa_K325219 |Part Main Page]]
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<span style="color:black">This part should show similarity to</span> [https://parts.igem.org/Part:BBa_K325219 BBa_K325219]&nbsp;&nbsp;&nbsp;&nbsp; [[Part:BBa_K325{{{id}}}:Experience |Add Data]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Part:BBa_K325219:ArabinosetoLight|Arabinose -> Light]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Part:BBa_K325219:LuciferintoLight|Luciferin -> Light]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Part:BBa_K325219:Stability |Effect of pH]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Part:BBa_K325219:Experience |Add Data]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</center>
 
</center>
 
</div><br>
 
</div><br>
  
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<div style="padding: 00px; width: 680px; border: 0px solid #000000;">
 
 
'''Description'''<br>
 
'''Description'''<br>
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This part is based on part [https://parts.igem.org/Part:BBa_K325219 BBa_K325219].  Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part [https://parts.igem.org/Part:BBa_K325209 BBa_K325209] which is the green wild-type luciferase].  Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.   
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This part is based on part [https://parts.igem.org/Part:BBa_K325219 BBa_K325219].  Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part [https://parts.igem.org/Part:BBa_K325209 BBa_K325209] which is the green wild-type luciferase.  Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.   
  
  
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==Pictures==
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==Colour of emission==
 +
 
 +
[[Image:Rainbow.png|thumb|569px|center|Figure 1 - Comparison of part BBa_K325{{{id}}} against other constructs created by the E.glowli team.  Note that this picture should be used as a relative comparison rather than absolute colour.  All images change markedly depending on the white balance used.]]
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[[Image:300px-Cambridge-Wed.jpg|thumb|569px|center|'''Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with [https://parts.igem.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [https://parts.igem.org/Part:BBa_K325219 BBa 325219] (red light bulb) ''']]
 
  
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</center>
 
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<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
 
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<div style="padding: 00px; width: 680px">
 
 
'''Compatibility'''<br>
 
'''Compatibility'''<br>
 
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br>
 
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br>
 
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
 
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
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</div>
 
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'''References'''<br>
 
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[http://www.ncbi.nlm.nih.gov/pubmed/18949818 '''[1&#x5d;:'''] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,''Life'' '''61''', 6-17.
 
−
</div>
 
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[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html '''[2&#x5d;:'''] T. Nakatsu ''et al.'' (2006) Structural Basis for the spectral difference in luciferase bioluminescence, ''Nature'' '''440'''(16), 372-376.
 
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[http://www.ncbi.nlm.nih.gov/pubmed/11457857 '''[3&#x5d;:'''] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, ''The Journal of Biological Chemistry'', '''276'''(39), 36508-36513.
 

Latest revision as of 01:04, 28 October 2010

Input: L-Arabinose
Output: Light ({{{colour}}})

pBad/araC
I0500		mutated version of
Luciferase/LRE
K325210

This part should show similarity to BBa_K325219     [[Part:BBa_K325{{{id}}}:Experience |Add Data]]       


Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.


Colour of emission

Figure 1 - Comparison of part BBa_K325{{{id}}} against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.


Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3