Difference between revisions of "Part:BBa K374006"
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In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. The nut site is a gene regulatory sequence situated upstream of the terminator, and consists of three more or less conserved regions the boxA, boxB and boxC regions[2]. The native ''E. coli'' regulatory protein NusA binds to the spacer region between the boxA and boxB, and interacts with the N protein that binds to boxB, the complex binds another NusA protein, and the complex prevents the formation of the terminator stem loop.[1] Antitermination has been reported in synthetic constructs when the nut site was situated 200bp upstream of the terminator [3]. | In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. The nut site is a gene regulatory sequence situated upstream of the terminator, and consists of three more or less conserved regions the boxA, boxB and boxC regions[2]. The native ''E. coli'' regulatory protein NusA binds to the spacer region between the boxA and boxB, and interacts with the N protein that binds to boxB, the complex binds another NusA protein, and the complex prevents the formation of the terminator stem loop.[1] Antitermination has been reported in synthetic constructs when the nut site was situated 200bp upstream of the terminator [3]. | ||
| − | This biobrick was submitted by the [http://2010.igem.org/Team:DTU-Denmark 2010 DTU iGEM team], see our wiki for further description of the parts and for an example of an application | + | This biobrick was submitted by the [http://2010.igem.org/Team:DTU-Denmark 2010 DTU iGEM team], see our wiki for further description of the parts and for an example of an application: Construction of a Bistable Switch. |
| − | === | + | === References === |
* [1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768. | * [1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768. | ||
* [2] Prasch. S., Jurk. M., Washburn. R.S., Gottesman. M.E., Wöhrl. B., Rösch. P., "Rna-binding specificity of E. coli NusA" Nucleic Acids Research 2009. | * [2] Prasch. S., Jurk. M., Washburn. R.S., Gottesman. M.E., Wöhrl. B., Rösch. P., "Rna-binding specificity of E. coli NusA" Nucleic Acids Research 2009. | ||
Latest revision as of 00:52, 28 October 2010
Lambda N anti-terminator.
This part contains the coding sequence for the lambda N anti terminator protein, which will suppress intrinsic transcription termination downstream of the nut site a regulatory sequence, see BBa_K374005.K374005 | |
 |
In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. The nut site is a gene regulatory sequence situated upstream of the terminator, and consists of three more or less conserved regions the boxA, boxB and boxC regions[2]. The native E. coli regulatory protein NusA binds to the spacer region between the boxA and boxB, and interacts with the N protein that binds to boxB, the complex binds another NusA protein, and the complex prevents the formation of the terminator stem loop.[1] Antitermination has been reported in synthetic constructs when the nut site was situated 200bp upstream of the terminator [3].
This biobrick was submitted by the [http://2010.igem.org/Team:DTU-Denmark 2010 DTU iGEM team], see our wiki for further description of the parts and for an example of an application: Construction of a Bistable Switch.
References
- [1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768.
- [2] Prasch. S., Jurk. M., Washburn. R.S., Gottesman. M.E., Wöhrl. B., Rösch. P., "Rna-binding specificity of E. coli NusA" Nucleic Acids Research 2009.
- [3] Whalen. W., Ghosh. B., Das. A., "NusA protein is necessary and sufficient in vitro for phage lambda N gene product to suppress a rho-independent terminator placed downstream of nutL. Proc.Natl. Acad sci. 1988
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]