Difference between revisions of "Part:BBa K374006"

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	<partinfo>BBa_K374006 short</partinfo>		<partinfo>BBa_K374006 short</partinfo>
−	In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. This part contains the lambda N gene which will suppress transcription termination downstream of part BBa_K374005.	+	This part contains the coding sequence for the lambda N anti terminator protein, which will suppress intrinsic transcription termination downstream of the nut site a regulatory sequence, see BBa_K374005.<partinfo>BBa_K374005 SpecifiedComponents</partinfo>
−	[1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768.	+	In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. The nut site is a gene regulatory sequence situated upstream of the terminator, and consists of three more or less conserved regions the boxA, boxB and boxC regions[2]. The native ''E. coli'' regulatory protein NusA binds to the spacer region between the boxA and boxB, and interacts with the N protein that binds to boxB, the complex binds another NusA protein, and the complex prevents the formation of the terminator stem loop.[1] Antitermination has been reported in synthetic constructs when the nut site was situated 200bp upstream of the terminator [3].
		+
		+	This biobrick was submitted by the [http://2010.igem.org/Team:DTU-Denmark 2010 DTU iGEM team], see our wiki for further description of the parts and for an example of an application: Construction of a Bistable Switch.
		+
		+	=== References ===
		+	* [1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768.
		+	* [2] Prasch. S., Jurk. M., Washburn. R.S., Gottesman. M.E., Wöhrl. B., Rösch. P., "Rna-binding specificity of E. coli NusA" Nucleic Acids Research 2009.
		+	* [3] Whalen. W., Ghosh. B., Das. A., "NusA protein is necessary and sufficient in vitro for phage lambda N gene product to suppress a rho-independent terminator placed downstream of nutL. Proc.Natl. Acad sci. 1988
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Latest revision as of 00:52, 28 October 2010

Lambda N anti-terminator.

This part contains the coding sequence for the lambda N anti terminator protein, which will suppress intrinsic transcription termination downstream of the nut site a regulatory sequence, see BBa_K374005.

In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. The nut site is a gene regulatory sequence situated upstream of the terminator, and consists of three more or less conserved regions the boxA, boxB and boxC regions[2]. The native E. coli regulatory protein NusA binds to the spacer region between the boxA and boxB, and interacts with the N protein that binds to boxB, the complex binds another NusA protein, and the complex prevents the formation of the terminator stem loop.[1] Antitermination has been reported in synthetic constructs when the nut site was situated 200bp upstream of the terminator [3].

This biobrick was submitted by the [http://2010.igem.org/Team:DTU-Denmark 2010 DTU iGEM team], see our wiki for further description of the parts and for an example of an application: Construction of a Bistable Switch.

References

• [1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768.
• [2] Prasch. S., Jurk. M., Washburn. R.S., Gottesman. M.E., Wöhrl. B., Rösch. P., "Rna-binding specificity of E. coli NusA" Nucleic Acids Research 2009.
• [3] Whalen. W., Ghosh. B., Das. A., "NusA protein is necessary and sufficient in vitro for phage lambda N gene product to suppress a rho-independent terminator placed downstream of nutL. Proc.Natl. Acad sci. 1988

Sequence and Features